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Development and characterization of microsatellite loci in Lamprotula leai, with cross-amplification in Hyriopsis cumingii

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Abstract

Eighteen microsatellite loci were isolated and characterized in Lamprotula leai. The number of alleles observed for each locus ranged from 3 to 13. The values of observed heterozygosity and expected heterozygosity ranged from 0.2083 to 0.8944 and from 0.4305 to 0.8961, respectively. Fifteen loci conformed to Hardy–Weinberg equilibrium and no linkage disequilibrium was found. A cross-amplification test showed ten out of eighteen loci were successfully amplified in three hyriopsis cumingii populations. These informative microsatellite markers will be useful to assess genetic diversity for this species.

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Acknowledgments

This study was supported by the Prophase Program of the Key Project of Chinese National Programs for Fundamental Research and Development (973 progranm) (2009CB126000), the National Natural Science Foundation of China (30871923).

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Authors and Affiliations

  1. Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, 201306, Shanghai, China

    Bo Xu, Jia-Le Li & Gui-Ling Wang

  2. Aquaculture Division, E-Institute of Shanghai Universities, Shanghai Ocean University, 201306, Shanghai, China

    Jia-Le Li

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  1. Bo Xu
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  2. Jia-Le Li
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  3. Gui-Ling Wang
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Correspondence to Gui-Ling Wang.

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Xu, B., Li, JL. & Wang, GL. Development and characterization of microsatellite loci in Lamprotula leai, with cross-amplification in Hyriopsis cumingii . Conservation Genet Resour 3, 545–547 (2011). https://doi.org/10.1007/s12686-011-9400-0

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  • DOI: https://doi.org/10.1007/s12686-011-9400-0

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