Abstract
Twenty-nine microsatellites were isolated from genomic DNA of spotted seatrout (Cynoscion nebulosus). The microsatellites were screened across 10–25 individuals from a single population sampled in the Lower Laguna Madre, Texas. All microsatellites were polymorphic with the number of alleles per locus ranging from 3 to 25. Expected heterozygosity ranged from 0.34 to 0.96. No deviations from Hardy–Weinberg equilibrium or linkage disequilibrium were observed following correction for multiple simultaneous tests.
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References
Anderson JD, Karel WJ (2010) Population genetics and dynamics of spotted seatrout in the estuarine waters of Texas. Fish Aquat J 2010:FAJ2
Barbara T, Palma-Silva C, Paggi GM, Bered F, Fay MF, Lexer C (2007) Cross-species of nuclear microsatellite markers: potential and limitations. Mol Ecol 16:3759–3767
Carson EW, Karlsson S, Saillant E, Gold JR (2009) Genetic studies of hatchery-supplemented populations of red drum in four Texas Bays. N Am J Fish Manage 29:1502–1510
Clark RD, Morrison W, Christensen JD, Monaco ME, Coyne MS (2003) Modeling the distribution and abundance of spotted seatrout: integration of ecology and GIS technology to support management needs. In: Bortone SA (ed) Biology of the spotted seatrout. CRC Press, Boca Raton, pp 247–266
Gold JR, Stewart LB, Ward R (2003) Population structure of spotted seatrout (Cynoscion nebulosus) along the Texas Gulf Coast, as revealed by genetic analysis. In: Bartone SA (ed) Biology of the spotted seatrout. CRC Press, Boca Raton, pp 31–40
Hutchings JA, Reynolds JD (2004) Marine fish population collapses: consequences for recovery and extinction. Bioscience 54:297–309
Karlsson S, Saillant E, Bumguardner BW, Vega RR, Gold JR (2008) Genetic identification of hatchery-released red drum in Texas Bays and estuaries. N Am J Fish Manage 28:1294–1304
Policansky D, Magnuson JJ (1998) Genetics, metapopulations, and ecosystem management of fisheries. Ecol Appl 8:S119–S123
Raymond M, Rousset F (1995) GenePop version 1.2: population genetics software for exact tests and ecumenicism. J Hered 86:248–249
Reddy OUK, Pepper AE, Abdurakhmonov I, Saha S, Jenkins JN, Brooks T, Bolek Y, El-Zik KM (2001) New dinucleotide and trinucleotide microsatellite marker resources for cotton genome research. J Cotton Sci 5:103–113
Riccioni G, Landi M, Ferrara G, Milano I, Cariani A, Zane L, Sella M, Barbujani G, Tinti F (2010) Spatio-temporal population structuring and genetic diversity retention in depleted Atlantic bluefin tuna of the Mediterranean Sea. Proc Nat Acad Sci 107:2102–2107
Rice W (1989) Analyzing tables of statistical tests. Evolution 43:223–225
Rousset F (2008) Genepop’007: a complete reimplementation of the GenePop software for Windows and Linus. Mol Ecol Res 8:103–106
Rozen S, Skaletsky HJ (2000) Primer3 on the WWW for general users and for biologist programmers. In: Krawetz S, Misener S (eds) Bioinformatics methods and protocols: methods in molecular biology. Humana Press, Totowa, pp 365–386
Schuelke M (2000) An economic method for the fluorescent labeling of PCR fragments. Nat Biotechnol 18:233–234
Ward R, Bowers K, Hensley R, Mobley B, Belouski E (2007) Genetic variability in spotted seatrout (Cynoscion nebulosus), determined with microsatellite DNA markers. Fish Bull 105:197–206
Acknowledgments
Financial support was provided by the San Antonio Chapter of the Saltwater Enhancement Association and Texas Parks and Wildlife Department, Texas A&M University-Corpus Christi, TRDF grant to Dr. R. Deborah Overath, and Coastal Conservation Association grant to Dr. Gregory W. Stunz. Samples were provided by Robert Adami of Texas Parks and Wildlife Department. We thank Dr. John R. Gold (Texas A&M University) for his critical review of this manuscript.
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Blandon, I.R., Morales, C., Vega, R.R. et al. Development of primers for 29 microsatellite loci in spotted seatrout (Cynoscion nebulosus). Conservation Genet Resour 3, 123–126 (2011). https://doi.org/10.1007/s12686-010-9305-3
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DOI: https://doi.org/10.1007/s12686-010-9305-3