Conservation Genetics Resources

, Volume 2, Issue 1, pp 223–226

Characterization of nine polymorphic microsatellite loci in Chorocaris sp. (Crustacea, Caridea, Alvinocarididae) from deep-sea hydrothermal vents

  • K. A. Zelnio
  • A. D. Thaler
  • R. E. Jones
  • W. Saleu
  • T. F. Schultz
  • C. L. Van Dover
  • J. Carlsson
Technical Note

DOI: 10.1007/s12686-010-9243-0

Cite this article as:
Zelnio, K.A., Thaler, A.D., Jones, R.E. et al. Conservation Genet Resour (2010) 2: 223. doi:10.1007/s12686-010-9243-0

Abstract

Species in the genus Chorocaris are caridean shrimp found at deep-sea hydrothermal vents at mid-ocean spreading centers. Nine unlinked polymorphic microsatellite loci were developed for this Chorocaris sp. 2 from the Manus back-arc basin, southwest Pacific. Three loci deviated significantly from Hardy–Weinberg expectations. Average observed heterozygosity ranged from 0.17 to 0.74 (mean HO = 0.45, SD = 0.21). Eight out of nine loci cross-amplified in one or two additional species of alvinocaridid shrimp that co-occur with Chorocaris sp. 2 at western Pacific vent habitats. The efficacy of our markers to detect genetic population structure is demonstrated using 362 individuals from 3 sites in Manus Basin. Microsatellites developed for Chorocaris sp. 2 are being deployed to study connectivity among populations of this species colonizing geographically discrete back-arc basin vent systems.

Keywords

Alvinocarididae Back-arc Basin Chemosynthetic-based ecosystem Chorocaris Hydrothermal vent Shrimp Microsatellite 

Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  • K. A. Zelnio
    • 1
  • A. D. Thaler
    • 1
  • R. E. Jones
    • 1
  • W. Saleu
    • 1
  • T. F. Schultz
    • 1
  • C. L. Van Dover
    • 1
  • J. Carlsson
    • 1
    • 2
  1. 1.Marine Conservation Molecular Facility, Marine Laboratory, Nicholas School of the EnvironmentDuke UniversityBeaufortUSA
  2. 2.Department of Zoology, Ecology and Plant ScienceUniversity College CorkCorkIreland