Abstract
We describe the isolation and development of 20 polymorphic microsatellite loci for the ornate dragon lizard Ctenophorus ornatus. Characterization of these loci in 29 individuals from a single population yielded between five and twenty alleles per locus. Expected heterozygosities ranged from 0.32 to 1.00 and only one locus deviated from Hardy–Weinberg expectations. There was no evidence of linkage disequilibrium between any pair of loci.
References
Goudet J (2001) FSTAT, a program to estimate and test gene diversities and fixation indices. (version 2.9.3). Available from http://www.unil.ch/izea/softwares/fstat.html (Updated from: Goudet J (1995) FSTAT (version 1.2): a computer program to calculate F-statistics. J Hered 86:485–486)
Jones KC, Levine KF, Banks JD (2002) Characterization of 11 polymorphic tetranucleotide microsatellites for forensic applications in California elk (Cervus elaphus canadensis). Mol Ecol Notes 2:425–427
LeBas NR (2001) Microsatellite determination of male reproductive success in a natural population of the territorial ornate dragon lizard, Ctenophorus ornatus. Mol Ecol 10:193–203
LeBas NR (2002) Mate choice, genetic incompatibility, and outbreeding in the ornate dragon lizard, Ctenophorus ornatus. Evolution 56:371–377
LeBas NR, Marshall NJ (2000) The role of colour in signalling and male choice in the agamid lizard Ctenophorus ornatus. Proc R Soc Lond B Biol Sci 267:445–452
LeBas NR, Marshall NJ (2001) No evidence of female choice for a condition-dependent trait in the agamid lizard, Ctenophorus ornatus. Behaviour 138:965–980
LeBas NR, Spencer PB (2000) Polymorphic microsatellite markers in the ornate dragon lizard, Ctenophorus ornatus. Mol Ecol 9:365–378
Marshall TC, Slate J, Kruuk LEB, Pemberton JM (1998) Statistical confidence for likelihood-based paternity inference in natural populations. Mol Ecol 7:639–655
Rice WR (1989) Analyzing tables of statistical tests. Evolution 43:223–225
Saunders DA (1989) Changes in the avifauna of a region, district and remnant as a result of fragmentation of native vegetation—the wheatbelt of Western Australia—a case-study. Biol Conserv 50:99–135
Storr GM, Smith LA, Johnstone RE (1983) Lizards of Western Australia II. Dragons and monitors. Western Australian museum, Western Australia
Sunnucks P, Hales DF (1996) Numerous transposed sequences of mitochondrial cytochrome oxidase I-II in aphids of the genus Sitobion (Hemiptera: Aphididae). Mol Biol Evol 13:510–524
Yates CJ, Ladd PG, Coates DJ, McArthur S (2007) Hierarchies of cause: understanding rarity in an endemic shrub Verticordia staminosa (Myrtaceae) with a highly restricted distribution. Aust J Bot 55:194–205
Acknowledgments
We thank Mark Todd from Genetic Identification Services for his helpful advice. This work was funded by an Australian Research Council grant to NRL.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Levy, E., Kennington, W.J. & LeBas, N.R. Characterization of microsatellite loci for the ornate dragon lizard Ctenophorus ornatus . Conservation Genet Resour 2 (Suppl 1), 313–315 (2010). https://doi.org/10.1007/s12686-010-9216-3
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s12686-010-9216-3