Technical Note

Conservation Genetics Resources

, Volume 1, Issue 1, pp 273-276

Characterisation of polymorphic microsatellite markers in the widespread Australian seagrass, Posidonia australis Hook. f. (Posidoniaceae), with cross-amplification in the sympatric P. sinuosa

  • E. A. SinclairAffiliated withBotanic Gardens & Parks AuthoritySchool of Plant Biology, University of Western Australia Email author 
  • , J. AnthonyAffiliated withBotanic Gardens & Parks Authority
  • , G. T. CouplandAffiliated withSchool of Plant Biology, University of Western Australia
  • , M. WaycottAffiliated withSchool of Marine and Tropical Biology, James Cook University
  • , M. D. BarrettAffiliated withBotanic Gardens & Parks AuthoritySchool of Plant Biology, University of Western Australia
  • , R. L. BarrettAffiliated withBotanic Gardens & Parks AuthoritySchool of Plant Biology, University of Western Australia
  • , M. L. CambridgeAffiliated withSchool of Plant Biology, University of Western Australia
  • , M. J. WallaceAffiliated withBotanic Gardens & Parks AuthoritySchool of Plant Biology, University of Western Australia
  • , K. W. DixonAffiliated withBotanic Gardens & Parks AuthoritySchool of Plant Biology, University of Western Australia
    • , S. L. KraussAffiliated withBotanic Gardens & Parks AuthoritySchool of Plant Biology, University of Western Australia
    • , G. A. KendrickAffiliated withSchool of Plant Biology, University of Western Australia

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Abstract

We developed 10 polymorphic microsatellite markers in the Australian seagrass Posidonia australis Hook. f. Markers were screened for their ability to detect within- and among-population genetic structure and variation. The markers showed a range in levels of polymorphism from fixed differences between the two sampled seagrass meadows to high levels of heterozygosity. These markers will be used to estimate gene flow across the species range, characterise the mating system through paternity analysis and pollen dispersal, characterise the nature and extent of clonality, and determine the genetic differentiation of local seagrass meadows to provide information on where to source local genetic provenance material for seagrass restoration projects. Seven of the 10 loci also amplified in the sympatric P. sinuosa and will be useful in future studies in population genetics and hybridisation.

Keywords

Posidonia australis Microsatellites Clonal diversity Restoration Cross-species amplification Posidonia sinuosa