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Effect of Adenoviral Transduction of Hepatic Stellate Cells with Adv5-optHGF-RFP on their Phenotype

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Abstract

Adenovirus is a promising gene delivery vector that has a high efficiency and relative simplicity of construction. These advantages make this system attractive for diverse research applications. In this project we transduced hepatics stellatecells (HSC) with adenoviral vector containing genes of hepatocytes growth factor (HGF) and red fluorescent protein (RFP) as a reporter (Adv5-optHGF-RFP), which let us to visualize the transduced cells. Further changes of phenotype were studied by real-time PCR and immunocytochemical staining. According to our results, most of the cells were transduced and demonstrated stable expression of RFP. Effectiveness of the transduction was also confirmed by a high expression of HGF (1200 times higher than in control HSC culture). Adenoviral transduction of HSC with HGF gene did not change their morphology, but stimulated expression of HSC marker desmin, leading to HSC activation (α-smooth muscle actin) and appearance of α-fetoprotein, one of the hepatoblasts markers. During the entire experiment, there were Ki-67+ cells, meaning that proliferation of transduced HSC was not affected. Thus, adenoviral transduction of HSC with Adv5-optHGF-RFP is a good gene delivery system with a stimulating effect on HSC.

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References

  1. Friedman, S. L. (2008). Hepatic stellate cells: protean, multifunctional, and enigmatic cells of the liver. Physiol Rev, 88, 125–172.

    Article  Google Scholar 

  2. Kordes, C., et al. (2007). CD133+ hepatic stellate cells are progenitor cells. Biochem Biophys Res Commun, 352(2), 410–417.

    Article  Google Scholar 

  3. Kiassov, A. P., et al. (1995). Desmin expressing nonhematopoietic liver cells during rat liver development: an immunohistochemical and morphometric study. Differentiation, 59(4), 253–258.

    Article  Google Scholar 

  4. Kordes, C., et al. (2014). Hepatic stellate cells contribute to progenitor cells and liver regeneration. J Clin Invest, 124(12), 5503–5515.

    Article  Google Scholar 

  5. Birchmeier, C., & Gherardi, E. (1998). Developmental roles of HGF/SF and its receptor, the c-Met tyrosine kinase. Trends Cell Biol, 8, 404–410.

    Article  Google Scholar 

  6. Oh, S. H., et al. (2000). Hepatocyte growth factor induces differentiation of adult rat bone marrow cells into a hepatocyte lineage in vitro. Biochem Biophys Res Commun, 279(2), 500–504.

    Article  Google Scholar 

  7. Snykers, S., et al. (2006). Sequential exposure to cytokines reflecting embryogenesis: the key for in vitro differentiation of adult bone marrow stem cells into functional hepatocyte-like cells. Toxicol Sci, 94(2), 330–341.

    Article  Google Scholar 

  8. Reetz, J., Genz, B., Meier, C., Kowtharapu, B. S., Timm, F., Vollmar, B., et al. (2013). Development of adenoviral delivery systems to target hepatic stellate cells in vivo. PLoS One, 8(6), e67091. doi:10.1371/journal.pone.0067091.

    Article  Google Scholar 

  9. Franceschi, R. T., & Ge, C. (2008). Gene delivery by adenoviruses. In J. J. Westendorf (Ed.), Osteoporosis. Methods and protocols (1st ed., pp. 137–147). Rochester, USA: Humana Press.

    Chapter  Google Scholar 

  10. Knook, D. L., Seffelaar, A. M., & de Leeuw, A. M. (1982). Fat-storing cells of the rat liver. Exp Cell Res, 132, 468–471.

    Article  Google Scholar 

  11. Nava, S., et al. (2005). Characterization of cells in the developing human liver. Differentiation, 73, 249–260.

    Article  Google Scholar 

Download references

Acknowledgements

The work is performed according to the Russian Government Program of Competitive Growth of Kazan Federal University and subsidy allocated to Kazan Federal University for the state assignment in the sphere of scientific activities and financial support by grant №12-04-97088 of the Russian Foundation for Basic Research.

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Authors and Affiliations

  1. Institute of Fundamental Medicine and Biology, Kazan Federal University, 74 Karl Marks str, Kazan, 420008, Russia

    Aygul Shafigullina, Elvira Zaikina, Ekaterina Garanina, Damir Sakhapov, Angelina Titova, Mikhail Mavlikeev, Albert Rizvanov, Anisa Gumerova & Andrey Kiassov

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  1. Aygul Shafigullina
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  2. Elvira Zaikina
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  3. Ekaterina Garanina
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  4. Damir Sakhapov
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  5. Angelina Titova
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  6. Mikhail Mavlikeev
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  7. Albert Rizvanov
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  8. Anisa Gumerova
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  9. Andrey Kiassov
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Correspondence to Aygul Shafigullina.

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The authors declare no conflicts of interest.

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Aygul Shafigullina and Elvira Zaikina are equally contributing authors

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Shafigullina, A., Zaikina, E., Garanina, E. et al. Effect of Adenoviral Transduction of Hepatic Stellate Cells with Adv5-optHGF-RFP on their Phenotype. BioNanoSci. 7, 419–422 (2017). https://doi.org/10.1007/s12668-016-0378-7

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  • DOI: https://doi.org/10.1007/s12668-016-0378-7

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