Skip to main content
SpringerLink
Log in

Liver Cells Proliferation and Apoptosis in Patients with Alcoholic Liver Disease After Autologous Hematopoietic Stem Cell Transplantation

  • Published:
BioNanoScience Aims and scope Submit manuscript

Abstract

Alcoholic liver disease is a huge medical and social problem that leads to liver fibrosis, cirrhosis, and hepatocellular carcinoma. Unfortunately, the last stages of the disease do not have efficient treatment, except liver transplantation, and require development of new therapeutical approaches. Transplantation of stem cells might be the most promising approach. In our research, we studied transplantation of autologous hematopoietic stem cells (HSC) into the celiac trunk of patients with alcoholic liver cirrhosis. In this article, we pay particular attention to proliferation and apoptosis—two fundamental processes, which determine the fate of regeneration. Liver biopsy specimens before treatment, 3 and 12 months after transplantation of HSC, were stained immunohistochemically with antibodies against PCNA and Bcl-2. The results showed that treatment was safe and effective, hepatocytes increased proliferation, and inflammatory cells decreased antiapoptotic activity, signifying improvement in liver regeneration. However, effect of treatment after 12 months decreases and requires repeated HSC transplantation.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

References

  1. Fujii, H., Hirose, T., Oe, S., et al. (2002). Contribution of bone marrow cells to liver regeneration after partial hepatectomy in mice. J Hepatol, 36(5), 653–659.

    Article  Google Scholar 

  2. Masson, S., Harrison, D. J., Plevvris, J. N., Newsome, P. H. (2004). Potential of hematopoietic stem cell therapy in hepatology: a critical review. Stem cells, 22(6), 897–907.

    Article  Google Scholar 

  3. Kakinuma, S., Tanaka, Y., Chinzei, R., et al. (2003). Human umbilical cord blood as a source of transplantable hepatic progenitor cells. Stem Cells, 21(2), 217–227.

    Article  Google Scholar 

  4. Tang, X. P., Yang, X., Tan, H., Ding, Y. L., Zhang, M., Wang, W. L. (2003). Clinical and experimental study on therapeutic effect of umbilical cord blood transplantation on severe viral hepatitis. World J. Gastroenterol, 9(9), 1999–2003.

    Article  Google Scholar 

  5. Am Esch, J. S., II, Knoefel, W. T., Klein, M., et al. (2005). Portal application of autologous CD133+ bone marrow cells to the liver: a novel concept to support hepatic regeneration. Stem Cells, 23(4), 463–470.

    Article  Google Scholar 

  6. Am Esch, J. S., II, Schmelzle, M., Fürst, G., et al. (2012). Infusion of CD133+ bone marrow-derived stem cells after selective portal vein embolization enhances functional hepatic reserves after extended right hepatectomy: a retrospective single-center study. Ann. Surg, 255(1), 79–85.

    Article  Google Scholar 

  7. Saadeh, S., Cammel, G., Carey, W. D., Younossi, Z., Barnes, D., Easley, K. (2001). The role of liver biopsy in chronic hepatitis C. Hepatology, 33(1), 196–200.

    Article  Google Scholar 

  8. Leonardi, E., Girlando, S., Serio, G., Mauri, F. A., Perrone, G., Scampini, S., et al. (1992). PCNA and Ki67 expression in breast carcinoma: correlations with clinical and biological variables. J Clin. Pathol, 45(5), 416–419.

    Article  Google Scholar 

  9. Vaux, D. L., Cory, S., Adams, J. M. (1988). Bcl-2 gene promotes haemopoietic cell survival and cooperates with c-myc to immortalize pre-B cells. Nature, 335, 440–442.

    Article  Google Scholar 

  10. Kim, J. K., Park, Y. N., Kim, J. S., et al. (2010). Autologous bone marrow infusion activates the progenitor cell compartment in patients with advanced liver cirrhosis. Cell Transplant, 19(10), 1237–1246.

    Article  Google Scholar 

  11. Terai, S., Sakaida, I., Yamamoto, N., et al. (2003). An in vivo model for monitoring transdifferentiation of bone marrow cells into functional hepatocytes. J. Biochem, 134(4), 551–558.

    Article  Google Scholar 

Download references

Acknowledgments

This work was performed as a part of the Program “Development of Cell Medicine in Republic of Tatarstan” on the base of the Republican Clinical Hospital and according to the Russian Government Program of Competitive Growth of Kazan Federal University and subsidy allocated to Kazan Federal University for the state assignment in the sphere of scientific activities.

Author information

Author notes

    Authors and Affiliations

    1. Department of Morphology and General Pathology, Kazan Federal University, 18 Kremlyovskaya st, Kazan, 420008, Russia

      Guzel Burganova, Sayar Abdulkhakov, Anisa Gumerova, Marina Titova & Andrey Kiassov

    2. Department of Endoscopy, Uniclinic of Kazan Federal University, 1a Chehova st, Kazan, 420043, Russia

      Guzel Burganova

    3. Department of Human Anatomy, Kazan State Medical University, 49 Butlerov st, Kazan, 420012, Russia

      Sayar Abdulkhakov & Ilnaz Gazizov

    4. Department of Gastroenterology, Republican Clinical Hospital, Ministry of Health, 138 Orenburgskii tract, Kazan, 420064, Russia

      Alfiya Odintcova

    Authors
    1. Guzel Burganova
      View author publications

      You can also search for this author in PubMed Google Scholar

    2. Sayar Abdulkhakov
      View author publications

      You can also search for this author in PubMed Google Scholar

    3. Ilnaz Gazizov
      View author publications

      You can also search for this author in PubMed Google Scholar

    4. Anisa Gumerova
      View author publications

      You can also search for this author in PubMed Google Scholar

    5. Marina Titova
      View author publications

      You can also search for this author in PubMed Google Scholar

    6. Alfiya Odintcova
      View author publications

      You can also search for this author in PubMed Google Scholar

    7. Andrey Kiassov
      View author publications

      You can also search for this author in PubMed Google Scholar

    Corresponding author

    Correspondence to Guzel Burganova.

    Ethics declarations

    This single center uncontrolled open-label clinical trial was approved by the Republican Ethical Committee of Ministry of Health of the Republic of Tatarstan. The study was performed after obtaining written informed consent form from all patients in accordance with ethical norms of Helsinki declaration (2000).

    Additional information

    Guzel Burganova, Sayar Abdulkhakov, Ilnaz Gazizov, Anisa Gumerova, Marina Titova, Alfiya Odintcova, and Andrey Kiassov contributed equally to this work.

    Rights and permissions

    Reprints and permissions

    About this article

    Check for updates. Verify currency and authenticity via CrossMark

    Cite this article

    Burganova, G., Abdulkhakov, S., Gazizov, I. et al. Liver Cells Proliferation and Apoptosis in Patients with Alcoholic Liver Disease After Autologous Hematopoietic Stem Cell Transplantation. BioNanoSci. 7, 302–305 (2017). https://doi.org/10.1007/s12668-016-0354-2

    Download citation

    • Published:

    • Issue Date:

    • DOI: https://doi.org/10.1007/s12668-016-0354-2

    Keywords

    Search

    Navigation