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Rat Bile Duct Decellularization

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Abstract

The repair and reconstruction of bile ducts damaged by disease or trauma remains a vexing medical problem. In particular, surgeons have few options when it comes to a long segment reconstruction of the bile duct. Tissue-engineered substitutes, with properties similar to the native tissue, might represent a solution for the functional reconstruction of bile ducts. In particular, decellularized tissues and organs represent a suitable option for tissue engineering when specific scaffolds are needed. However, the optimal conditions to completely remove all the cellular components and minimally affect the structural and residual biochemical properties of the extracellular matrix are still to be found. This paper presents the characterization of rat bile ducts after implementing an established detergent-enzymatic decellularization method. One cycle was enough to generate a complete decellularized bile duct matrix, histologically and structurally similar to the native one. The network of collagen, reticular and elastic fibers, found in the native bile duct matrix was well preserved. Moreover, the decellularization approach did not affect the elastin content.

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Acknowledgments

This work was supported by a grant (pd 239-28/04/2009, GRT 1210/08) issued on 28 December 2008 by the region Tuscany (Italy) entitled “Clinical laboratory for complex thoracic respiratory and vascular diseases and alternatives to pulmonary transplantation.”

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Authors and Affiliations

  1. BIOAIRLab, University Hospital Careggi, Florence, Italy

    Silvia Baiguera

  2. Laboratory of Bioengineering and Regenerative Medicine (BioReM), Kazan (Volga Region) Federal University, Kazan, Russia

    Svetlana Arkhipva & Paolo Macchiarini

  3. Animal Microsurgery Center, Departments of Surgery, Biological Science Division, University of Chicago, Chicago, IL, USA

    Dengping Yin

  4. University of Illinois College of Medicine Peoria, Illinois, USA

    Mark Holterman

Authors
  1. Silvia Baiguera
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  2. Svetlana Arkhipva
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  3. Dengping Yin
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  4. Mark Holterman
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  5. Paolo Macchiarini
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Corresponding author

Correspondence to Paolo Macchiarini.

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The publication of this manuscript was approved by the Institutional Review Board of the Kazan Federal University. The study was approved by the Animal Care and Use Committee and the Bioethics Committee of the University of Florence (Italy).

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Baiguera, S., Arkhipva, S., Yin, D. et al. Rat Bile Duct Decellularization. BioNanoSci. 6, 578–584 (2016). https://doi.org/10.1007/s12668-016-0287-9

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  • DOI: https://doi.org/10.1007/s12668-016-0287-9

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