Abstract
Plants harbor homologues of various animal genes involved in phosphorus metabolism, telomere biology and other cellular processes. Compared to experiments with many other multicellular organisms, research in the model plant Arabidopsis thaliana takes advantage of short generation time and an ever increasing arsenal of genetic and transgenic tools, including large collections of T-DNA knockout and activation lines. The availability of thousands of publicly available transgenic Arabidopsis lines provides a unique opportunity to address a number of important biological questions. However, identification of individual T-DNA mutant plants from a pool of seeds provided by a biological stock distribution center remains a laborious and time-consuming procedure. Here we compared a number of commercial Taq DNA polymerases commonly used for routine PCR genotyping to identify a single polymerase most suitable for genotyping T-DNA mutant plants. Our data indicate that Emerald Amp GT PCR Master Mix provides the most reliable, quick and simple DNA genotyping tool to determine the presence of a T-DNA insertion and to establish whether an individual A. thaliana plant is heterozygous or homozygous for the mutant allele.
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Acknowledgments
We thank Xiaoyuan Xie, Callie Kobayashi and other members of the Dorothy Shippen laboratory (Texas A&M University) for help with setting up large-scale PCR assays. This work was supported by the National Institutes of Health (grant number 1R03AG052891-01 to EVS) and Russian Foundation for Basic Research (grant number 15-04-01645 to EVS). LRN was supported in part by the Foundation for the Promotion of Innovation (Small Innovative Enterprises in Science and Technology Assistance Fund, UMNIK project №0020895). This work was performed in accordance with the Russian Government Program of Competitive Growth of Kazan Federal University.
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Chastukhina, I.B., Nigmatullina, L.R., Valeeva, L.R. et al. Selection of Efficient Taq DNA Polymerase to Optimize T-DNA Genotyping Method for Rapid Detection of Mutant Arabidopsis thaliana Plants. BioNanoSci. 6, 407–410 (2016). https://doi.org/10.1007/s12668-016-0253-6
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DOI: https://doi.org/10.1007/s12668-016-0253-6