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Cloning and Heterologous Expression of Phytase Gene from Pantoea sp. 3.5.1

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Abstract

Phytases (myo-inositol hexakisphosphate phosphohydrolase) catalyzes the stepwise hydrolysis of phosphate groups from phytic acid (myo-inositol hexakisphosphate) or its salt phytate. These enzymes could be potentially used for the stereospecific and efficient production of different myo-inositol phosphate isomers with therapeutic features. In the present study, we cloned the 1728 bp open reading frame encoding Pantoea sp. 3.5.1 phytase into the expression vector pET28a. The recombinant Escherichia coli BL21 pLysS pET28a/agpP strain expressing Pantoea sp. 3.5.1 AgpP phytase was obtained.

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Acknowledgments

This work was supported by the Russian Foundation for Basic Research (project no. 16-34-60191) and by the subsidy allocated to Kazan Federal University for the state assignment in the sphere of scientific activities (project no. 14–83 0211/02.11.10083.001). The work is performed according to the Russian Government Program of Competitive Growth of Kazan Federal University.

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  1. Kazan (Volga region) Federal University, Kazan, Russia

    Aliya D. Suleimanova, Inna B. Chastukhina, Liia R. Valeeva, Darja L. Itkina & Margarita R. Sharipova

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  1. Aliya D. Suleimanova
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  2. Inna B. Chastukhina
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  3. Liia R. Valeeva
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  4. Darja L. Itkina
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  5. Margarita R. Sharipova
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Correspondence to Aliya D. Suleimanova.

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Suleimanova, A.D., Chastukhina, I.B., Valeeva, L.R. et al. Cloning and Heterologous Expression of Phytase Gene from Pantoea sp. 3.5.1. BioNanoSci. 6, 338–340 (2016). https://doi.org/10.1007/s12668-016-0227-8

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  • DOI: https://doi.org/10.1007/s12668-016-0227-8

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