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The Morphological Changes in the Internal Organs in Tumor-Bearing Rats at Intravenous Injection of Citrate-Stabilized Magnetite Nanoparticles

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Abstract

The usefulness of magnetite nanoparticles to improve contrast in magnetic resonance imaging (MRI) of tumor visualization was actively investigated currently. In this work, we conducted MRI and morphological examination of internal organs of 30 white outbred rats with transplanted liver tumor PC-1 after intravenous administration of magnetite hydrosols. Intravenous administration of citrate-stabilized magnetite nanoparticles in a dosage of 20 μg/kg and 16 mg/kg resulted in a disruption of blood filling of the organs, mainly due to the plethora. The dystrophic cell damage was observed in liver and in kidneys. The activation of immune processes in spleen was fixed after intravenous administration of citrate stabilized magnetite nanoparticles. In both doses, the nanoparticles were founded in the heart and brain, but the significant iron accumulation was detected in the tumor by methods of MRI and atomic absorption spectroscopy (AAS) only in dosage of 16 mg/kg. It is possible to conclude that selected dosage is sufficient for accumulation of nanoparticles in tumor, but it does not cause significant changes in the internal organs.

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Acknowledgments

The work was supported by the Government of the Russian Federation (grant no.14.Z50.31.0004 to support scientific research projects implemented under the supervision of leading scientists at Russian institutions and Russian institutions of higher education) and the Russian Scientific Foundation (project no. 14-15-00128) and Saratov State University. The work done by NAN was supported by the Russian President’s Scholarship SP-523.2016.4 (2016-2018).

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Authors and Affiliations

  1. Saratov Medical State University, 410012, Saratov, B. Kazachaya Str. 112, Russia

    Nikita A. Navolokin, Alla B. Bucharskaya, Victor V. Zuev, Olga S. Godage, Galina N. Maslyakova & Georgy S. Terentyuk

  2. Saratov State University, 410012, Saratov, Astrakhanskaya Str. 83, Russia

    Sergey V. German, Georgy S. Terentyuk & Dmitry A. Gorin

Authors
  1. Nikita A. Navolokin
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  2. Alla B. Bucharskaya
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  3. Sergey V. German
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  4. Victor V. Zuev
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  5. Olga S. Godage
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  6. Galina N. Maslyakova
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  7. Georgy S. Terentyuk
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  8. Dmitry A. Gorin
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Correspondence to Alla B. Bucharskaya.

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Navolokin, N.A., Bucharskaya, A.B., German, S.V. et al. The Morphological Changes in the Internal Organs in Tumor-Bearing Rats at Intravenous Injection of Citrate-Stabilized Magnetite Nanoparticles. BioNanoSci. 6, 162–168 (2016). https://doi.org/10.1007/s12668-016-0200-6

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