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Pediocin-Loaded Nanoliposomes and Hybrid Alginate–Nanoliposome Delivery Systems for Slow Release of Pediocin

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Abstract

Pediocin is small antilisterial polypeptide bacteriocin. Direct addition of pediocin has been studied so far and is found to be effective only for a limited duration of time. The controlled release or slow release is an efficient method to prolong the effectiveness of pediocin and other such antimicrobials. Encapsulation is one of the numerous strategies that can be employed for achieving controlled release. Diverse wall materials such as phospholipids, proteins and carbohydrate polymers and combinations of these are used to encapsulate active ingredients. One of the aims of this study was to evaluate the effect of process parameters, viz. concentration of phospholipid (lecithin, phophatidylcholine) at the rate of 0.1, 0.2 and 0.3 % (w/v), amplitude for sonication (40, 50 and 60 %) and sonication time (3, 5 and 7 min), on size of nanoliposomes. There was an increase in liposome size with increase in concentration of phospholipids; however, there was decrease in size with increase in amplitude and duration of sonication. The main aim of the study was to compare the controlled release behaviour of pediocin loaded in delivery systems of different wall materials, viz. nanoliposomes (lecithin, phophatidylcholine), capsules of alginate plus guar gum and hybrid capsules of alginate plus guar gum incorporated with nanoliposomes. Encapsulated pediocin was found to be more effective in inhibiting bacterial growth than directly added pediocin. Hybrid capsules of alginate plus guar gum incorporated with pediocin-loaded nanoliposomes of phosphatidylcholine were found to be the best delivery system for controlled release of pediocin.

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Acknowledgments

This work was supported by National Fund for Basis, Strategic and Frontier Application Research in Agriculture, Indian Council of Agricultural Research, New Delhi, through project entitled “Microencapsulation methods for bacteriocins for their controlled release” (Project code no. PHT-2003).

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Authors and Affiliations

  1. Central Institute of Post-harvest Engineering and Technology (CIPHET), Ludhiana, India

    K. Narsaiah, S. N. Jha, Robin A. Wilson & H. M. Mandge

  2. FG&OP Division, Central Institute of Post-harvest Engineering and Technology (CIPHET), Ludhiana, India

    M. R. Manikantan

  3. Dairy Microbiology Division, National Dairy Research Institute, Karnal, India

    R. K. Malik & S. Vij

Authors
  1. K. Narsaiah
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  2. S. N. Jha
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  3. Robin A. Wilson
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Correspondence to K. Narsaiah.

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Narsaiah, K., Jha, S.N., Wilson, R.A. et al. Pediocin-Loaded Nanoliposomes and Hybrid Alginate–Nanoliposome Delivery Systems for Slow Release of Pediocin. BioNanoSci. 3, 37–42 (2013). https://doi.org/10.1007/s12668-012-0069-y

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