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Enriched Flavonoid Fraction from Cecropia pachystachya Trécul Leaves Exerts Antidepressant-like Behavior and Protects Brain Against Oxidative Stress in Rats Subjected to Chronic Mild Stress

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Abstract

The purpose of this study was to assess the effect of an enriched C-glycosyl flavonoids fraction (EFF-Cp) from Cecropia Pachystachya leaves on behavior, mitochondrial chain function, and oxidative balance in the brain of rats subjected to chronic mild stress. Male Wistar rats were divided into experimental groups (saline/no stress, saline/stress, EFF-Cp/no stress, and EFF-Cp/stress). ECM groups were submitted to stress for 40 days. On the 35th ECM day, EFF-Cp (50 mg/kg) or saline was administrated and the treatments lasted until the 42nd day. On the 41st and 42nd days, the animals were submitted to the splash test and the forced swim test. After these behavioral tests, the enzymatic activity of mitochondrial chain complexes and oxidative stress were analyzed. EFF-Cp reversed the depressive-like behavior induced by ECM. It also reversed the increase in thiobarbituric acid reactive species, myeloperoxidase activity, and nitrite/nitrate concentrations in some brain regions. The reduced activities of the antioxidants superoxide dismutase and catalase in some brain regions were also reversed by EFF-Cp. The most pronounced effect of EFF-Cp on mitochondrial complexes was an increase in complex IV activity in all studied regions. Thus, it is can be concluded that EFF-Cp exerts an antidepressant-like effect and that oxidative balance may be an important physiological process underlying these effects.

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Acknowledgments

Laboratory of Neurosciences (Brazil) is a center within the National Institute for Translational Medicine (INCT-TM) and a member of the Center of Excellence in Applied Neurosciences of Santa Catarina (NENASC). This research was supported by grants from CNPq (JQ and GZR), FAPESC (JQ), Instituto Cérebro e Mente, and UNESC (JQ and ELS). JQ, FHR, and ELS are CNPq Research Fellows. C.F. ORTMANN is also grateful to CAPES for their research fellowship. Center for Translational Psychiatry (USA) is funded by Department of Psychiatry and Behavioral Sciences, The University of Texas Medical School at Houston.

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  1. Programa de Pós-graduação em Farmácia, Universidade Federal de Santa Catarina, Florianópolis, SC, Brazil

    Caroline F. Ortmann, Pâmela de Carvalho, Angela M. de Campos & Flávio H. Reginatto

  2. Laboratório de Neurociências, Programa de Pós-Graduação em Ciências da Saúde, Unidade Acadêmica de Ciências da Saúde, Universidade do Extremo Sul Catarinense, Criciúma, SC, 88806-000, Brazil

    Gislaine Z. Réus, Zuleide M. Ignácio, Helena M. Abelaira, Camila O. Arent, Maria Augusta B. dos Santos, Beatriz I. Matias & João Quevedo

  3. Department of Psychiatry and Behavioral Sciences, Center for Translational Psychiatry, The University of Texas Medical School at Houston, Houston, TX, USA

    Stephanie E. Titus & João Quevedo

  4. Laboratório de Fisiopatologia Clínica e Experimental, Programa de Pós-graduação em Ciências da Saúde, Universidade do Sul de Santa Catarina, Tubarão, SC, Brazil

    Maryane M. Martins & Fabricia Petronilho

  5. Laboratório de Bioenergética, Programa de Pós-Graduação em Ciências da Saúde, Unidade Acadêmica de Ciências da Saúde, Universidade do Extremo Sul Catarinense, Criciúma, SC, Brazil

    Leticia J. Teixeira, Meline O. S. Morais & Emilio L. Streck

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Correspondence to Gislaine Z. Réus.

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Ortmann, C.F., Réus, G.Z., Ignácio, Z.M. et al. Enriched Flavonoid Fraction from Cecropia pachystachya Trécul Leaves Exerts Antidepressant-like Behavior and Protects Brain Against Oxidative Stress in Rats Subjected to Chronic Mild Stress. Neurotox Res 29, 469–483 (2016). https://doi.org/10.1007/s12640-016-9596-6

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