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Safety of Lactobacillus plantarum ST8Sh and Its Bacteriocin

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Abstract

Total DNA extracted from Lb. plantarum ST8Sh was screened for the presence of more than 50 genes related to production of biogenic amines (histidine decarboxylase, tyrosine decarboxylase, and ornithine decarboxylase), virulence factors (sex pheromones, gelatinase, cytolysin, hyaluronidase, aggregation substance, enterococcal surface protein, endocarditis antigen, adhesion of collagen, integration factors), and antibiotic resistance (vancomycin, tetracycline, erythromycin, gentamicin, chloramphenicol, bacitracin). Lb. plantarum ST8Sh showed a low presence of virulence genes. Only 13 genes were detected (related to sex pheromones, aggregation substance, adhesion of collagen, tetracycline, gentamicin, chloramphenicol, erythromycin, but not to vancomycin, and bacitracin) and may be considered as indication of safety for application in fermented food products. In addition, interaction between Lb. plantarum ST8Sh and drugs from different groups were determined in order to establish possible application of the strain in combination with commercial drugs. Cytotoxicity of the semi-purified bacteriocins produced by Lb. plantarum ST8Sh was depended on applied concentration—highly cytotoxic when applied at 25 μg/mL and no cytotoxicity at 5 μg/mL.

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Acknowledgements

This work has been supported by research and personal grants from FAPEMIG (Belo Horizonte, MG, Brazil), CAPES (Brasilia, DF, Brazil), and CNPq (Brasilia, DF, Brazil). To Braga A.C.S. and Batista M.N. from UNESP — Universidade Estadual Paulista, Instituto de Biociências, Letras e Ciências Exatas. Departamento de Engenharia e Tecnologia de Alimentos, São José do Rio Preto, SP, Brazil for technical assistance related to cytotoxicity of bacteriocin work.

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Authors and Affiliations

  1. Veterinary Department, Universidade Federal de Viçosa, Campus UFV, Viçosa, Minas Gerais, 36570-900, Brazil

    Svetoslav Dimitrov Todorov, Luana M. Perin & Luís Augusto Nero

  2. Departamento de Engenharia e Tecnologia de Alimentos, UNESP - Universidade Estadual Paulista, Instituto de Biociências, Letras e Ciências Exatas, São José do Rio Preto, SP, Brazil

    Bruno M. Carneiro & Paula Rahal

  3. Instituto de Ciências Exatas e Naturais, Universidade Federal de Mato Grosso, Rondonópolis, MT, Brazil

    Bruno M. Carneiro

  4. GEE, Handong Global University, Pohang, South Korea

    Wilhelm Holzapfel

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  1. Svetoslav Dimitrov Todorov
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  2. Luana M. Perin
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Correspondence to Svetoslav Dimitrov Todorov.

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Todorov, S.D., Perin, L.M., Carneiro, B.M. et al. Safety of Lactobacillus plantarum ST8Sh and Its Bacteriocin. Probiotics & Antimicro. Prot. 9, 334–344 (2017). https://doi.org/10.1007/s12602-017-9260-3

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