Skip to main content

Advertisement

SpringerLink
Log in

Optimization of Culture Conditions for Mass Production of the Probiotics Pseudomonas MCCB 102 and 103 Antagonistic to Pathogenic Vibrios in Aquaculture

  • Published:
Probiotics and Antimicrobial Proteins Aims and scope Submit manuscript

Abstract

Rapid growth of shrimp farming industry is affected by the recurrence of diverse diseases, among which vibriosis is predominant. Eco-friendly disease management strategy by the application of antagonistic probiotics is widely accepted. In the present study, culture conditions of antagonistic probiotics, Pseudomonas MCCB 102 and 103, were optimized to enhance their biomass production and antagonistic activity against the shrimp pathogen V. harveyi MCCB 111. Primarily, one-dimensional screening was carried out to fix the optimum range of sodium chloride concentration, pH and temperature. The second step optimization was done using a full-factorial central composite design of response surface methodology. As per the model, 12.9 g/L sodium chloride and pH 6.5 for Pseudomonas MCCB 102, and 5 g/L sodium chloride and pH 7 for Pseudomonas MCCB 103 were found to be ideal to maximize antagonistic activity. However, optimum temperature was the same (25 °C) for both isolates. Finally, the models were experimentally validated for enhanced biomass production and antagonistic activity. The optima for biomass and antagonistic activity were more or less the same, suggesting the possible influence of biomass on antagonistic activity.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

References

  1. Baron SS, Rowe JJ (1981) Antibiotic action of pyocyanin. Antimicrob Agents Chemother 20:814–820. doi:10.1128/AAC.20.6.814

    Article  CAS  Google Scholar 

  2. Bly JE, Quiniou SMA, Lawson LA, Clem LW (1997) Inhibition of Saprolegnia pathogenic for fish by Pseudomonas fluorescens. J Fish Dis 20:35–40. doi:10.1046/j.1365-2761.1997.d01-104.x

    Article  Google Scholar 

  3. Chin-A-Woeng TFC, Bloemberg GV, Lugtenberg BJJ (2003) Phenazines and their role in biocontrol by Pseudomonas bacteria. New Phytol 157:503–523. doi:10.1046/j.1469-8137.2003.00686.x

    Article  CAS  Google Scholar 

  4. Chythanya R, Karunasagar I, Karunasagar I (2002) Inhibition of shrimp pathogenic vibrios by a marine Pseudomonas I-2 strain. Aquaculture 208:1–10. doi:10.1016/S0044-8486(01)00714-1

    Article  Google Scholar 

  5. Esser DW, Eberly L, Hadero A, Crawford IP (1990) Identification and characterization of genes for a second anthranilate synthase in Pseudomonas aeruginosa: interchangeability of the anthranilate synthase and evolutionary implications. J Bacteriol 172:884–900

    Google Scholar 

  6. Gram L, Melchiorsen J, Spanggaard B, Huber I, Nielsen TF (1999) Inhibition of Vibrio anguillarum by Pseudomonas flurescens AH2, a possible probiotic treatment of fish. Appl Environ Microbiol 65:969–973

    CAS  Google Scholar 

  7. Guerra NP, Pastrana L (2002) Nisin and Pediocin production on mussel-processing waste supplemented with glucose and five nitrogen sources. Lett Appl Microbiol 34:114–118. doi:10.1046/j.1472-765x.2002.01054.x

    Article  CAS  Google Scholar 

  8. Jayaprakash NS (2005) Antibody based diagnostics for detection of vibrios and their biological control using antagonistic bacteria in Macrobrachium rosenbergii larval rearing system. Ph.D. Thesis, Cochin University of Science and Technology, India

  9. Jayaprakash NS, Somnath Pai S, Anas A, Preetha R, Philip R, Singh ISB (2006) A marine Micrococcus MCCB104 antagonistic to vibrios in prawn larval rearing systems. Dis Aquat Org 8:39–45

    Google Scholar 

  10. Kerr JK, Taylor GW, Rutman A, Hoiby N, Cole PJ, Wilson R (1999) Pseudomonas aeruginosa pyocyanin and 1-hydroxyphenazine inhibit fungal growth. J Clin Pathol 52:385–387

    Article  CAS  Google Scholar 

  11. Kumar RS, Ayyadurai N, Pandiaraja P, Reddy AV, Venkateswarlu Y, Prakash O, Sakthivel N (2005) Characterization of antifungal metabolite produced by a new strain Pseudomonas aeruginosa PUPa3 that exhibits broad-spectrum antifungal activity and biofertilizing traits. J Appl Microbiol 98:145–154. doi:10.1111/j.1365-2672.2004.02435.x

    Article  CAS  Google Scholar 

  12. Liong MT, Shah NP (2005) Optimization of cholesterol removal by probiotics in the presence of prebiotics by using a response surface method. Appl Environ Microbiol 71:1745–1753. doi:10.1128/AEM.71.4.1745-1753.2005

    Article  CAS  Google Scholar 

  13. Luis-Villasenor IE, Castellanos-Cervantes T, Gomez-Gil B, Carrillo-García AE, Campa-Córdova AI, Ascencio F (2013) Probiotics in the intestinal tract of juvenile whiteleg shrimp Litopenaeus vannamei: modulation of the bacterial community. World J Microbiol Biotechnol 29:257–265. doi:10.1007/s11274-012-1170-0

    Article  CAS  Google Scholar 

  14. Mavrodi DV, Blankenfeldt W, Thomashow LS (2006) Phenazine compounds in fluorescent Pseudomonas spp. biosynthesis and regulation. Annu Rev Phytopathol 44:18.1–18.29

    Article  Google Scholar 

  15. Norman RS, Moeller P, Mc Donald TJ, Morris PJ (2004) Effect of pyocyanin on a crude-oil-degrading microbial community. Appl Environ Microbiol 70:4004–4011. doi:10.1128/AEM.70.7.4004-4011.2004

    Article  CAS  Google Scholar 

  16. Preetha R, Jayaprakash NS, Philip R, Singh ISB (2007) Optimization of carbon and nitrogen sources and growth factors for the production of an aquaculture probiotic (Pseudomonas MCCB 103) using response surface methodology. J Appl Microbiol 102:1043–1051. doi:10.1111/j.1365-2672.2006.03149.x

    CAS  Google Scholar 

  17. Preetha R, Seena J, Prathapan S, Vijayan KK, Jayaprakash NS, Philip R, Singh ISB (2010) An inhibitory compound produced by Pseudomonas with effectiveness on Vibrio harveyi. Aquac Res 41:1452–1461. doi:10.1111/j.1365-2109.2009.02436.x

    CAS  Google Scholar 

  18. Raaijmakers JM, David MW, Thomasshow LS (1997) Frequency of antibiotic-producing Pseudomonas spp. in natural environment. Appl Environ Microbiol 63:881–887

    CAS  Google Scholar 

  19. Sen R, Swaminathan T (1997) Application of response-surface methodology to evaluate the optimum conditions for the enhanced production of surfactin. Appl Microbiol Biotechnol 47:358–363

    Article  CAS  Google Scholar 

  20. Smith P, Davey S (1993) Evidence for the competitive exclusion of Aeromonas salmonicida from fish with stress-inducible furunculosis by a fluorescent pseudomonad. J Fish Dis 16:521–524

    Article  Google Scholar 

  21. Vijayan KK, Singh ISB, Jayaprakash NS, Alavandi SV, Pai SS, Preetha R, Rajan JJS, Santiago TC (2006) A brackish water isolate of Pseudomonas PS-102, a potential antagonistic bacterium against pathogenic vibrios in penaeid and non-penaeid rearing systems. Aquaculture 251(192):200. doi:10.1016/j.aquaculture.2005.10.010

    Google Scholar 

  22. Wang Y-X, Lu Z-X (2004) Statistical optimization of media for extracellular polysaccharide by Pholiota squarrosa (Pers. Ex Fr.) Quel. AS 5.245 under submerged cultivation. Biochem Eng J 20:39–47

    Article  CAS  Google Scholar 

  23. Weuster-Botz D (2000) Experimental design for fermentation media development statistical design or global random search? J Biosci Eng 90:473–483. doi:10.1016/S1389-1723(01)80027-X

    CAS  Google Scholar 

Download references

Acknowledgments

This research was supported by Department of Ocean Development (DOD), Government of India [DOD/11/MRDF/4/3/UNI/97 (p-8)]. The first author thanks DOD for Fellowship.

Conflict of interest

R. Preetha, K.K. Vijayan, N.S. Jayapraksh, S.V. Alavandi, T.C. Santiago and I.S. Bright Singh declare that they have no conflict of interests.

Author information

Author notes

  1. R. Preetha

    Present address: Department of Food and Process Engineering, School of Bioengineering, SRM University, Kattankulathur, 603203, Tamil Nadu, India

Authors and Affiliations

  1. National Centre for Aquatic Animal Health, Cochin University of Science and Technology, Lakeside Campus, Fine Arts Avenue, Cochin, 682016, Kerala, India

    R. Preetha, N. S. Jayapraksh & I. S. Bright Singh

  2. Central Marine Fisheries Research Institute (Indian Council of Agriculture Research), Ernakulam North, Cochin, 682018, Kerala, India

    K. K. Vijayan

  3. Central Institute of Brackishwater Aquaculture (Indian Council of Agriculture Research), Chennai, 600028, Tamil Nadu, India

    S. V. Alavandi & T. C. Santiago

Authors
  1. R. Preetha
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. K. K. Vijayan
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. N. S. Jayapraksh
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. S. V. Alavandi
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. T. C. Santiago
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. I. S. Bright Singh
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to I. S. Bright Singh.

Electronic supplementary material

Below is the link to the electronic supplementary material.

Supplementary material 1 (DOC 50 kb)

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Preetha, R., Vijayan, K.K., Jayapraksh, N.S. et al. Optimization of Culture Conditions for Mass Production of the Probiotics Pseudomonas MCCB 102 and 103 Antagonistic to Pathogenic Vibrios in Aquaculture. Probiotics & Antimicro. Prot. 7, 137–145 (2015). https://doi.org/10.1007/s12602-015-9185-7

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12602-015-9185-7

Keywords

Search

Navigation