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Antimicrobial Effect of Lactobacillus reuteri on Cariogenic Bacteria Streptococcus gordonii, Streptococcus mutans, and Periodontal Diseases Actinomyces naeslundii and Tannerella forsythia

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Abstract

Lactic acid bacteria (LAB) are well known for their beneficial effects on human health in the intestine and immune system; however, there are few studies on the impact they can generate in oral health. The aim of this study was to test and compare in vitro antimicrobial activity of L. reuteri on pathogenic bacteria involved in the formation of dental caries: S. mutans, S. gordonii, and periodontal disease: A. naeslundii and T. forsythia. Also, we determined the growth kinetics of each bacterium involved in this study. Before determining the antimicrobial action of L. reuteri on cariogenic bacteria and periodontal disease, the behavior and cell development time of each pathogenic bacterium were studied. Once the conditions for good cell growth of each of selected pathogens were established according to their metabolic requirements, maximum exponential growth was determined, this being the reference point for analyzing the development or inhibition by LAB using the Kirby Bauer method. Chlorhexidine 0.12 % was positive control. L. reuteri was shown to have an inhibitory effect against S. mutans, followed by T. forsythia and S. gordonii, and a less significant effect against A. naeslundii. Regarding the effect shown by L. reuteri on the two major pathogens, we consider its potential use as a possible functional food in the prevention or treatment of oral diseases.

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Acknowledgments

This work was partly funded by a Grant from the Ministry of Public Education of Mexico, under the PROMEP program, Registration No. UANL-PTC-367 and by the Support Program for Scientific and Technological Research (PAICYT-UANL) No. CN 802-11. We particularly thank Suarez-Martínez VR for technical assistance in managing the anaerobic chamber. Dr. Med. Cardenas-Estrada E (CIDICS), who performed the statistical analysis of this study.

Conflict of interest

M. L. Baca-Castañón, M. A. De la Garza-Ramos, A. G. Alcázar-Pizaña, Y. Grondin, A. Coronado-Mendoza, R. I. Sánchez-Najera, E. Cárdenas-Estrada, C. E. Medina-De la Garza and E. Escamilla-García declare that they have no conflict of interests.

Author information

Authors and Affiliations

  1. Unidad de Odontología Integral y Especialidades, Centro de Investigación y Desarrollo en Ciencias de la Salud (CIDICS), Universidad Autónoma de Nuevo León (UANL), Ave. Carlos Canseco s/n con Ave. Gonzalitos, Mitras Centro, CP: 64460, Monterrey, Nuevo León, Mexico

    Magda Lorena Baca-Castañón, Myriam Angélica De la Garza-Ramos, Andrea Guadalupe Alcázar-Pizaña, Anahí Coronado-Mendoza & Erandi Escamilla-García

  2. Unidad de Ensayos Clínicos, Centro de Investigación y Desarrollo en Ciencias de la Salud (CIDICS), Universidad Autónoma de Nuevo León (UANL), Ave. Carlos Canseco s/n con Ave. Gonzalitos, Mitras Centro, CP: 64460, Monterrey, Nuevo León, Mexico

    Eloy Cárdenas-Estrada

  3. Unidad de Inmunomoduladores, Centro de Investigación y Desarrollo en Ciencias de la Salud (CIDICS), Universidad Autónoma de Nuevo León (UANL), Ave. Carlos Canseco s/n con Ave. Gonzalitos, Mitras Centro, CP: 64460, Monterrey, Nuevo León, Mexico

    Carlos Eduardo Medina-De la Garza

  4. Departamento de Microbiología, Facultad de Odontología, Universidad Autónoma de Nuevo León (UANL), Monterrey, Nuevo León, Mexico

    Magda Lorena Baca-Castañón, Myriam Angélica De la Garza-Ramos, Andrea Guadalupe Alcázar-Pizaña, Rosa Isela Sánchez-Najera & Erandi Escamilla-García

  5. Departamento de Inmunología, Facultad de Medicina, Universidad Autónoma de Nuevo León, Monterrey, Nuevo León, Mexico

    Carlos Eduardo Medina-De la Garza

  6. Facultad de Ciencias Biológicas, Universidad Autónoma de Nuevo León, San Nicolás de los Garza, Nuevo León, Mexico

    Yohann Grondin

Authors
  1. Magda Lorena Baca-Castañón
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  2. Myriam Angélica De la Garza-Ramos
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  8. Carlos Eduardo Medina-De la Garza
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  9. Erandi Escamilla-García
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Correspondence to Erandi Escamilla-García.

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Magda Lorena Baca-Castañón and Myriam Angélica De la Garza-Ramos are first authors.

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Baca-Castañón, M.L., De la Garza-Ramos, M.A., Alcázar-Pizaña, A.G. et al. Antimicrobial Effect of Lactobacillus reuteri on Cariogenic Bacteria Streptococcus gordonii, Streptococcus mutans, and Periodontal Diseases Actinomyces naeslundii and Tannerella forsythia . Probiotics & Antimicro. Prot. 7, 1–8 (2015). https://doi.org/10.1007/s12602-014-9178-y

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