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Immune Response of Salmonella Challenged Broiler Chickens Fed Diets Containing Gallipro®, a Bacillus subtilis Probiotic

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Abstract

This study was conducted to investigate the effect of feeding a probiotic, Bacillus subtilis, on antibody titers against Newcastle and infectious bursal viruses in broiler chickens challenged with Salmonella enterica serotype Enteritidis. One hundred and sixty 1-day-old broiler chicks were randomly assigned to four treatments in a completely randomized design. The treatments were negative control, probiotic-treated group, challenged group, and challenged probiotic treated group. Salmonella challenging decreased (P < 0.05) the relative weights of spleen and bursa. Inclusion of probiotic to diet of challenged chickens increased the relative weight of spleen, but had no effect on the relative weight of bursa. There were no differences for the antibody titers of chickens between negative control and probiotic-treated group. Salmonella challenging decreased (P < 0.05) antibody titers against Newcastle and infectious bursal viruses. Improvements in the antibody titers were observed by the addition of probiotic to diet of these chickens. The results showed that dietary inclusion of probiotic had no significant effect on immune parameters of chickens at non-contaminated environment, display a greater efficacy at environment contaminated with pathogen and can improve immune responses of infected chickens.

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Acknowledgments

The authors are grateful to the Islamic Azad University for research funding support, to the Agricultural, Medical and Industrial Research School, NSTRI, for supplying the basal diet and allowing the use of their poultry unit, and to the Biochem Company (Zusatzstoffe GmbH, Germany) for providing a sample of the probiotic Gallipro®. We also thank all of the staff members at the poultry unit for the assistance in the care and feeding of the chickens used in this research.

Conflict of interest

Ali Asghar Sadeghi, Parvin Shawrang and Shirin Shakorzadeh declare that there is no conflict of interest.

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Authors and Affiliations

  1. Department of Animal Science, Science and Research Branch, Islamic Azad University, Tehran, Iran

    Ali Asghar Sadeghi & Shirin Shakorzadeh

  2. Nuclear Agriculture Research School, Nuclear Science and Technology Research Institute, AEOI, Karaj, Iran

    Parvin Shawrang

Authors
  1. Ali Asghar Sadeghi
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  2. Parvin Shawrang
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  3. Shirin Shakorzadeh
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Correspondence to Ali Asghar Sadeghi.

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Sadeghi, A.A., Shawrang, P. & Shakorzadeh, S. Immune Response of Salmonella Challenged Broiler Chickens Fed Diets Containing Gallipro®, a Bacillus subtilis Probiotic. Probiotics & Antimicro. Prot. 7, 24–30 (2015). https://doi.org/10.1007/s12602-014-9175-1

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  • DOI: https://doi.org/10.1007/s12602-014-9175-1

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