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Characterization and Antilisterial Effect of Phosphatidylcholine Nanovesicles Containing the Antimicrobial Peptide Pediocin

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Abstract

Encapsulation may provide increased stability and antimicrobial efficiency to bacteriocins. In this work, the antilisterial peptide pediocin was encapsulated in nanovesicles prepared from partially purified soybean phosphatidylcholine. The maintenance of antimicrobial activity and properties of free and encapsulated pediocin was observed during 13 days at 4 °C, and after this period, the encapsulated pediocin retained 50 % its initial activity. The maintenance of the bioactive properties of free and encapsulated pediocin was observed against different species of Listeria, inhibiting Listeria monocytogenes, Listeria innocua and Listeria ivanovii. The size of vesicles containing pediocin was determined by dynamic light scattering as an average of 190 nm, with little change throughout the observation period. Polydispersity index values were around 0.201 and are considered satisfactory, indicating an adequate size distribution of liposomes. The efficiency of encapsulation was 80 %. Considering these results, the protocol used was appropriate for the encapsulation of this bacteriocin. Results demonstrate the production of stable nanoparticulate material. The maintenance of the properties of pediocin encapsulated in liposomes is fundamental to prospect the stability in different conditions of the food matrix.

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Acknowledgments

Authors thank to Dr. Sylvia Stanisçuaski Guterres from UFRGS, for the use of the Zetasizer equipment and to Center of Electronic Microscopy (UFRGS) for technical support. This research was financially supported by CNPq, Brazil. We declare that there is no conflict of interest.

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Authors and Affiliations

  1. Laboratório de Inspeção de Produtos de Origem Animal, Departamento de Veterinária Preventiva, Faculdade de Veterinária, Universidade Federal de Pelotas, Pelotas, Brazil

    Michele Brauner de Mello

  2. Laboratório de Microbiologia e Bioquímica Aplicada, Instituto de Ciência e Tecnologia de Alimentos, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil

    Patrícia da Silva Malheiros & Adriano Brandelli

  3. Laboratório de Instrumentação e Dinâmica Molecular, Instituto de Química, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil

    Nádya Pesce da Silveira

  4. Inspeção e Tecnologia de Leite e Derivados, Ovos e Mel. Departamento de Veterinária Preventiva, Faculdade de Veterinária, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil

    Márcia Monks Jantzen

  5. Instituto de Ciências Básicas da Saúde, Departamento de Microbiologia, Imunologia e Parasitologia, Universidade Federal do Rio Grande do Sul, Rua Sarmento Leite 500, sala 158, 90050-170, Porto Alegre, Brazil

    Amanda de Souza da Motta

Authors
  1. Michele Brauner de Mello
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  2. Patrícia da Silva Malheiros
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  3. Adriano Brandelli
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  4. Nádya Pesce da Silveira
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  5. Márcia Monks Jantzen
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  6. Amanda de Souza da Motta
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Correspondence to Amanda de Souza da Motta.

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de Mello, M.B., da Silva Malheiros, P., Brandelli, A. et al. Characterization and Antilisterial Effect of Phosphatidylcholine Nanovesicles Containing the Antimicrobial Peptide Pediocin. Probiotics & Antimicro. Prot. 5, 43–50 (2013). https://doi.org/10.1007/s12602-013-9125-3

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  • DOI: https://doi.org/10.1007/s12602-013-9125-3

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