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Detection of mefenoxam-resistant strains of Peronospora belbahrii, the causal agent of basil downy mildew, transmitted through infected seeds

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Abstract

Epidemics of basil downy mildew (DM) incited by Peronospora belbahrii have been very severe in Italy since 2013, in part due to the very favorable weather conditions, and losses have occurred in many commercial farms, even after repeated mefenoxam treatments. DM populations from basil plants and seeds, which are associated with failure in downy mildew control under field and greenhouse conditions, have been tested for their sensitivity to mefenoxam. Basil plants were inoculated with a sporangial suspension of seven DM populations and treated, before and after inoculation with the pathogen, with different dosages of mefenoxam: 100 μg/ml, which corresponds to the currently applied field dosage, 200 μg/ml and 1000 μg/ml. Azoxystrobin was used at the field dosage as the chemical control. Three out of four DM populations from seeds and two out of three from basil plants were found to be able to infect basil plants in the presence of 100 μg/ml and 200 μg/ml of mefenoxam, while the field dosage of azoxystrobin (186 μg/ml) was found to be completely effective. The sensitive populations of P. belbahrii were completely controlled by the field dosage of both chemicals also 14 days after the last treatment. This study provides new information on the potential risk of introducing mefenoxam-resistant P. belbahrii inoculum in the field through seeds infected by resistant strains.

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Acknowledgments

The research leading to these results has received funding from the European Union’s Horizon 2020 research and innovation programme under grant agreement No 634179 “Effective Management of Pests and Harmful Alien Species - Integrated Solutions” (EMPHASIS). The authors kindly thank Marguerite Jones for the language revision and the anonymous referees for the very helpful revision.

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Authors and Affiliations

  1. Centre for Innovation in the Agro-Environmental Sector, AGROINNOVA, University of Torino, Largo P. Braccini 2, 10095, Grugliasco, TO, Italy

    Ilenia Pintore, Giovanna Gilardi, Maria Lodovica Gullino & Angelo Garibaldi

  2. Department of Agricultural, Forest and Food Sciences (DISAFA), University of Torino, Largo P. Braccini 2, 10095, Grugliasco, TO, Italy

    Giovanna Gilardi & Maria Lodovica Gullino

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  1. Ilenia Pintore
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  2. Giovanna Gilardi
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  3. Maria Lodovica Gullino
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  4. Angelo Garibaldi
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Correspondence to Giovanna Gilardi.

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Pintore, I., Gilardi, G., Gullino, M.L. et al. Detection of mefenoxam-resistant strains of Peronospora belbahrii, the causal agent of basil downy mildew, transmitted through infected seeds. Phytoparasitica 44, 563–569 (2016). https://doi.org/10.1007/s12600-016-0538-x

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  • DOI: https://doi.org/10.1007/s12600-016-0538-x

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