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Present status of Citrus tristeza virus infecting Citrus spp. in Darjeeling hills and its detection in different plant parts

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Abstract

Darjeeling hills of West Bengal (India) are well known for the production of mandarin orange (Citrus reticulata). The spread of Citrus tristeza virus (CTV) in this area has threatened the cultivation of citrus. Therefore, a study of the incidence and distribution of CTV in Darjeeling hills was undertaken and a geographical map was prepared. In DAS-ELISA, a high titre of CTV was recorded in the tender shoot bark, leaf petiole and mid-rib of seven Citrus spp. Three citrus species, viz., pomelo, trifoliate and kumquat, were found to be free of CTV infection when tested in double antibody sandwich-enzyme linked immunosorbent assay (DAS-ELISA) and reverse transcription polymerase chain reaction (RT-PCR).

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Acknowledgments

The authors are grateful to the Joint Director (Research), IARI, and the Director, IARI, New Delhi for providing financial support.

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Author notes

  1. K. B. Pun

    Present address: Regional Rainfed Lowland Rice Research Station, Central Rice Research Institute, Gerua, 781 102, Assam, India

  2. R. Kumar

    Present address: Indian Agricultural Research Institute, Regional Station, Karnal, 132 001, Haryana, India

Authors and Affiliations

  1. Indian Agricultural Research Institute, Regional Station, Kalimpong, 734 301, West Bengal, India

    Amalendu Ghosh, Amrita Das, K. B. Pun, R. Kumar & Ramu Meena

  2. Advanced Centre for Plant Virology, Indian Agricultural Research Institute, New Delhi, 110 012, India

    V. K. Baranwal

Authors
  1. Amalendu Ghosh
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  2. Amrita Das
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  3. K. B. Pun
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  4. R. Kumar
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  5. Ramu Meena
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  6. V. K. Baranwal
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Correspondence to Amrita Das.

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Ghosh, A., Das, A., Pun, K.B. et al. Present status of Citrus tristeza virus infecting Citrus spp. in Darjeeling hills and its detection in different plant parts. Phytoparasitica 42, 381–386 (2014). https://doi.org/10.1007/s12600-013-0374-1

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  • DOI: https://doi.org/10.1007/s12600-013-0374-1

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