Abstract
A modified technique for the preparation of chromosomes from the different stages of meiosis from male grasshopper (Gesonula punctifrons) has been standardized. This technique is simpler and quicker method in comparison to squash technique. Moreover, this technique is inexpensive with low maintenance cost. With this method, a large number of dividing cells from testis of one animal can be accumulated. This technique will be of immense value in cytogenetic monitoring of grasshopper chromosomes for clear morphology and banding profiles.
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References
Arana, P., N. Henriques Gil, and J.L. Santos. 1987. Orientation behaviour of interchanges forming chiasma in interstitial regions: A cytological approach. Heredity 58: 15–24.
Banerjee, S.N., and S. Chakrabarti. 1989. Heteromorphic sex chromosome in Indian water frog Rana cyanophlyctis with notes on the distribution of C-band positive constitutive heterochromatin. Genetica Polonica 30(3): 177–180.
Brat, V.S., and S.K. Sethi. 1974. Polymorphism for meiotic characters in grasshopper. The Nucleus 17(i): 28–32.
Cabrero, J., A. Bugrov, E. Warchalowska-Sliwa, M.D. Lopez-Leon, F. Perfectti, and J.P.M. Camacho. 2003. Comparative FISH analysis in five species of Eyprepocnemidine grasshoppers. Heredity 90: 377–381.
Chakrabarti, S., S.N. Banerjee, L.N. Neogi, and S. Roychowdhury. 1983. C-band positive: W-chromosome in female Indian frog Rana tigrina. Experientia 39: 321–322.
Levan, A., K. Fredga, and A. Sandberg. 1964. Nomenclature for centromeric position on chromosomes. Hereditas 52: 201–220.
Paradeda, M.G., A. Bentos, and A. Novello. 2008. Comparative study of the grasshopper Zoniopoda tarsata (Romaleide) and Paulinia acuminata (Pauliniidae) based on chromosome and C-banding analysis. Caryologia 61(2): 123–127.
Mondal, N.K., and S. Chakrabarti. 2007. A simpler, cheaper and quicker method to study somatic chromosomes from Goat, Capra hircus. Cytologia 72(4): 419–425.
Sarkar, I. 1955. A translocation heterozygote in the grasshopper: Gesonula punctifrons. Journal of Heredity 46(4): 157–160.
Sumner, A.T. 1972. A simple technique for demonstrating centromeric heterochromatin. Experimental Cell Research 75: 304–306.
Acknowledgments
The Paper is dedicated to late Professor Samar Chakrabarti, Cancer Cytogenetics Unit, Department of Zoology, Burdwan University. The author is grateful to Prof. Ajoy Ray, Retired Professor in Zoology, Government College of West Bengal for inspiration and encouragements. He is also grateful to Prof. Asoke K. Pal, Department of Anatomy, Mahatma Gandhi Institute of Medical Sciences, Sebagram, Wardha for giving Giemsa stain as gift. The author is thankful to Ms.Soma Banerjee, Shri Shiksyatan School, Kolkata for support and help. The author is also thankful to Dr. Saswati Dasgupta, Teacher—in—Charge and Associate Professor in Physics, Rammohan College, Kolkata—700009 for support and encouragements.
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Banerjee, S.N. Modified Flame Drying Technique to Study Chromosomes of Grasshopper’s Testis. Proc Zool Soc 68, 217–221 (2015). https://doi.org/10.1007/s12595-014-0116-5
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DOI: https://doi.org/10.1007/s12595-014-0116-5