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Standardization of Genomic DNA Isolation from Minute Quantities of Fish Scales and Fins Amenable to RAPD-PCR

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Abstract

The main focus of this study was to standardize a non-destructive procedure for extraction of genomic DNA (gDNA) from minute quantities of scales and fins of two commonly available fishes Labeo bata and Heteropneustes fossilis and also to compare the gDNA yields from live and as well from frozen samples. The spectrophotometric and electrophoretic analyses revealed a significant difference in the DNA yields from live and frozen samples. The isolated gDNAs were used as templates for RAPD-PCR. The quality and consistency of banding pattern showed that gDNA templates from live tissues performed better than that from frozen tissue samples. It was also found that the minute quantities of fresh scales or fin tissues from live fish provided satisfactory quantity and quality of gDNAs that could support several rounds of RAPD-PCR. This non-destructive sampling has a great implication in gDNA based population genetic studies in endangered and vulnerable species of fishes, where killing or sacrificing is an ethical issue.

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Acknowledgments

The work was supported by a research grant from University Grants Commission (UGC), India [Sanction No.: 40-289/2011 (SR)] awarded to corresponding author.

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Authors and Affiliations

  1. Cell and Molecular Biology Laboratory, Department of Zoology, University of North Bengal, P.O. North Bengal University, Raja Rammohunpur, Siliguri, District Darjeeling, 734 013, West Bengal, India

    Tanmay Mukhopadhyay & Soumen Bhattacharjee

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  1. Tanmay Mukhopadhyay
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  2. Soumen Bhattacharjee
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Correspondence to Soumen Bhattacharjee.

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Mukhopadhyay, T., Bhattacharjee, S. Standardization of Genomic DNA Isolation from Minute Quantities of Fish Scales and Fins Amenable to RAPD-PCR. Proc Zool Soc 67, 28–32 (2014). https://doi.org/10.1007/s12595-013-0065-4

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  • DOI: https://doi.org/10.1007/s12595-013-0065-4

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