Skip to main content

Advertisement

SpringerLink
Log in

Effects of acetamiprid on immune system in female wistar rats

  • Research Article
  • Published:
Proceedings of the Zoological Society Aims and scope Submit manuscript

Abstract

A subacute toxicity study of acetamiprid was undertaken in 72 female wistar rats in four groups (18 each). Three different concentrations of acetamiprid (25, 100 and 200 mg/kg of body weight) were administered orally to rats. Untreated rat served as control. The antibody titre of sheep red blood cells (SRBC) in all the treated groups and decrease in cell mediated immune response (CMI) were evaluated by delayed type hypersensitivity reaction to dinitroflurobenzene (DNFB). Our results revealed that in 200 mg/kg body treated rats, there were significant (Pd≤0.01) decrease in mean values of total leukocyte count and relative lymphocyte count in rats. Globulin was also decreased significantly (Pd≤0.01) in acetamiprid treated rats compared to control. There was a significant decrease (Pd≤0.01) in spleen weight in rats treated with 200 mg/kg dose as compared to control. Histopathological examination of spleen revealed depletion of lymphocytes from Malpighian corpuscles in all treated groups in a dose dependent manner. The results indicated that acetamiprid suppressed both CMI and antibody forming ability of lymphocytes.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Benjamin, M. M. 1978. Outline of Veterinary Clinical Pathology. 3rd Edition. The Iowa State University Press, Iowa USA.

    Google Scholar 

  • Cha, S. W., Gu, H. K., Lee, K. P., Lee, M. H., Han, S. S., Jeong, T. C. 2000. Immunotxicity of ethyl carbamate in female BALB/c mice: role of esterase and cytochrome P-450. Toxicol. Lett. 115: 173–181.

    Article  CAS  PubMed  Google Scholar 

  • Clifford, J. I., Rees, K. R. 1967. The action of aflatoxin B1 on the rat liver. Biochem. J. 102: 65–75.

    CAS  PubMed  Google Scholar 

  • Culling, C. F. A. 1963. Handbook of histopathological and histochemical techniques. 3rd edition. Butter Worths and Co. (publishers), Ltd., London.

    Google Scholar 

  • Desai, I., Doobronyi, I., Vaga, L. 1986. Immuno-neuro and general Toxicologic animal studies on a synthetic pyrethroid: cypermethrin. Ecotoxicol. Environ. Safety. 12: 220–232.

    Article  Google Scholar 

  • Duncan, J. R., Prasse, K. W., Mahaffey, E. A. 1994. Vet. Lab. Med. (Clin. Path.). 3rd eds. Iowa State University Press. Iowa.

    Google Scholar 

  • Faith, R. E., Luster, M. I., Vos, L. G. 1980. Effect of immunocompetence by chemicals of environmental concern. Rev. Biochem. Toxicol. 2: 173–212.

    CAS  Google Scholar 

  • Forman, H. J., Thomas, M. J. 1986. Oxidant production and bactericidal activity in phagocytes. Annu. Rev. Physiol. 48: 669.

    Article  CAS  PubMed  Google Scholar 

  • Galloway, T., Handy, R. 2003. Immunotoxicity of organophosphorus pesticide. Ecotoxicol. 12: 325–363.

    Article  Google Scholar 

  • Gatne, M. M., Ramesh, Bhoir, P. S., Deore, M. D. 2006. Immunotoxicity studies of imidacloprid in rats. Toxicol. Int. 13(2): 89–92.

    CAS  Google Scholar 

  • Ghosh, R. C., Chauhan, H. V. S. 1991. Suppression of humoral immunity by purified aflatoxin B1 in broiler chiks. Indian journal of animal science. 61(1): 19–23.

    Google Scholar 

  • Gupta, R. P., Verma, P. C. 1998. Effect of gentamicin administration on certain clinico-pathological and mineral studies in guinea pigs. Indian Journal of veterinary pathology. 22(2): 123–126.

    Google Scholar 

  • Hogg, J. C. 1987. Neutrophil kinetics and lung injury. Physiol. Rev. 67: 1249.

    CAS  PubMed  Google Scholar 

  • Hudson, L., Hay, F. C. 1989. Practical immunology. 3rd Edn. K. M. Verghese Co., New Delhi.

    Google Scholar 

  • Jain, N. C. 1986. Schalm’s Veterinary Hematology, 4th Edn. Lea and Febiger, 600 Washington Square, Philadelphia, PA 19106-4198, USA.

    Google Scholar 

  • Kacmar, P., Pistl, J., Mikula, I. 1999. Immunotoxicology and veterinary medicine. Acta vet. Brno. 68: 57–79.

    Google Scholar 

  • Kaushal, V., Sharma, S., Brar, A. P. S., Soni, G. 2007. NDEA induced oxidative stress in albino rats-impact of dietary protein level. Toxicol. Int. 14(1): 33–39.

    CAS  Google Scholar 

  • Khurana, R., Chauhan, R. S. 2000. Immunopathological effects of fenvalarate on cell mediated immune response in sheep. J. Immunol. Immunopathol. 2: 56–59.

    Google Scholar 

  • Khurana, R., Chauhan, R. S. 2003. Immunopathological effects of monocrotophos on humoral immune response in sheep. J. Immunol. Immunopathol. 5(1): 54–56.

    Google Scholar 

  • Khurana, R., Mahipal, S. K., Chauhan, R. S. 1998. Effect of carbofuran on cell mediated immunity in sheep. Indian J. Toxicol. 5(2): 1–6.

    Google Scholar 

  • Kumar, R., Singhal, L. K., Singh, B. P., Chauhan, R. S. 2002. Effect of butachlor on cell-mediated immunity in chicken. J. Immunol. Immunopathol. 4(1): 84–87.

    Google Scholar 

  • LaFarge, C., Frayssinet, C. 1970. The reversibility of inhibition of RNA and DNA synthesis induced by aflatoxin in rat liver. A tentative explanation for carcinogenic mechanism. International journal of cancer. 6: 74–83.

    Article  CAS  Google Scholar 

  • Patel, B. J. 1996. Clinico-pathological and Immunological studies on cypermethrin toxicity in calves and laboratory animals. Ph. D. Thesis submitted to G. B. Pant University of Agriculture and Technology, Pantnagar (U. P.)

  • Paul, W. E. 1993. Infectious diseases and the immune system. Sci. Am. P-90.

  • Platt, W. R. 1979. Colour Atlas and Text book of haematology. J. B. Lippincott Co., Philadelphia.

    Google Scholar 

  • Prater, M. R. 2003. Immunotoxicity of dermal permethrin and cis-urocanic acid: effects of chemical mixtures in environmental health. Ph. D. thesis submitted to Virginia Polytechnic Institute and State University. Virginia.

  • Ragothaman, R. 1991. Toxicopathology of cypermethrin in rats. M.V.Sc. Thesis submitted to Tamil Nadu University and animal Sciences. Chenai.

  • Snedecor, G. W., Cochran, W. G. 1989. Statistical methods. 8th Edn. Iowa State University Press, Ames, Iowa.U.S.A. pp. 593.

    Google Scholar 

  • Staub, N. C. 1994. Pulmonary intravascular macrophages. Annu. Rev. Physiol. 56:47.

    Article  CAS  PubMed  Google Scholar 

  • Talwar, G. P. 1983. A handbook of practical immunology. Vikas Publishing House Pvt. Ltd., New Delhi.

    Google Scholar 

  • Tamang, R. K. 1987. Comparative pathology of pyrithroid and organophosphate pesticide intoxication in mice and goats. M.V.Sc. Thesis submitted to Birsa Agricultural university, Ranchi, Bihar.

  • Tamang, R. K., Jha, G. J., Gupta, M. K., Chauhan, H. V., Tiwari, B. K. 1988. In vivo immunosuppression by synthetic pyrethroid (cypermethrin) pesticide in mice and goats. Vet. Immunol. Immunopathol. 19(3): 299–305.

    Article  CAS  PubMed  Google Scholar 

  • Thaxton, J. P., Thung, H. T., Hamilton, P. B. 1974. Immunosuppressions in chickens by aflatoxin. Poultry science. 53: 721–725.

    CAS  PubMed  Google Scholar 

  • Toivanen, P., Toivanen, A., Good, R. A. 1972. Ontogeny of bursal function in chickens. F. Embryonic stem cell for humoral immunity. Journal of Immunology. 109: 1058–1070.

    CAS  Google Scholar 

  • Tomizawa, M., Casida, J. E. 2005. Neonicotinoid insecticide toxicology: mechanisms of selective action. Annual Review of Pharmacology and Toxicology. 45: 247–268.

    Article  CAS  PubMed  Google Scholar 

  • Varshneya, C., Singh, T., Sharma, L. D., Bahga, H. S., Garg, S. K. 1992. Immunotoxic responses of cypermethrin, a synthetic pyrethroid insecticide in rats. Indian J. Physiol. Pharmacol. 36(2): 123–126.

    CAS  PubMed  Google Scholar 

  • Young, J. D. 1989. Killing of target cells by lympocytes: A mechanistic review. Physiol. Rev. 69: 250.

    CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Veterinary Pathology, College of Veterinary Science and Animal Husbandry, Durg, 491 001, Chhattisgarh, India

    R. C. Ghosh & M. S. Mate

  2. Chembiotek, TCGLS, Kolkata, 700091, India

    Samiran Mondal & D. B. Karmakar

Authors
  1. Samiran Mondal
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. R. C. Ghosh
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. M. S. Mate
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. D. B. Karmakar
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Samiran Mondal.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Mondal, S., Ghosh, R.C., Mate, M.S. et al. Effects of acetamiprid on immune system in female wistar rats. Proc Zool Soc 62, 109–117 (2009). https://doi.org/10.1007/s12595-009-0012-6

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12595-009-0012-6

Keywords

Search

Navigation