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Distribution of three algicidal Alteromonas sp. strains in seagrass beds and surrounding areas in the Seto Inland Sea, Japan

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  • Environment
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Abstract

Harmful algal blooms (HABs) often cause great damage to the fish aquaculture industry in the western part of Japan. Bacteria that effectively kill such phytoplankton are found in coastal seawater and are considered to influence the occurrence of HABs. Algicidal bacteria are found in abundance in seagrass beds; however, the distribution of particular bacterial strains has not yet been clarified. In this study, we determined the abundance of three algicidal Alteromonas sp. strains that were isolated as Chattonella antiqua-killing bacteria in seagrass beds and their surrounding areas in summers of 2013–2015. The strains were detected using a quantitative polymerase chain reaction (qPCR) amplification method. Two of the three algicidal bacterial strains were remarkably abundant in the seawater of an enclosed seagrass bed compared to the surrounding areas. In addition, the abundance of the three algicidal bacteria decreased when the eelgrass withered in late summer. These results suggest that growth of these algicidal bacteria was stimulated by eelgrass. The bacterial abundance estimated by qPCR was much greater than that determined by the culture-dependent method in June 2015 when the eelgrass grew thickly. This implies that the qPCR assay could be a sensitive tool to evaluate algicidal bacteria in natural environments.

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Acknowledgements

We are grateful to Dr. I. Yoshinaga of the Tottori University of Environmental Studies, Mr. T. Katayama of Ocean Construction, and Mr. S. Kojima for their helpful advice and cooperation in the study. This study was supported financially by the Agriculture, Forestry, and Fisheries Research Council.

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Authors and Affiliations

  1. Tohoku National Fisheries Research Institute, Fisheries Research Agency, Shinhama-cho 3-27-5, Shiogama, Miyagi, 985-0001, Japan

    Tomoko Sakami & Nobuharu Inaba

  2. National Research Institute of Fisheries and Environment of Inland Sea, Fisheries Research Agency, Maruishi 2-17-5, Hatsukaichi, Hiroshima, 739-0452, Japan

    Setsuko Sakamoto

  3. Okayama Prefectural Office, Uchisange 2-4-6, Kita-ku, Okayama, 700-8570, Japan

    Shuzo Takagi

  4. Graduate School of Fisheries Science, Hokkaido University, Minato-cho 3-1-1, Hakodate, Hokkaido, 041-8611, Japan

    Ichiro Imai

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  1. Tomoko Sakami
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  2. Setsuko Sakamoto
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  4. Nobuharu Inaba
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  5. Ichiro Imai
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Correspondence to Tomoko Sakami.

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Sakami, T., Sakamoto, S., Takagi, S. et al. Distribution of three algicidal Alteromonas sp. strains in seagrass beds and surrounding areas in the Seto Inland Sea, Japan. Fish Sci 83, 113–121 (2017). https://doi.org/10.1007/s12562-016-1048-y

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  • DOI: https://doi.org/10.1007/s12562-016-1048-y

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