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Molecular cloning and expression analysis of Relish gene from the ridgetail white prawn Exopalaemon carinicauda

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Abstract

Rel/NF-κB transcription factors play critical roles in induction and regulation of innate immune response in organisms. In this study, the full length of a Relish homolog cDNA from Exopalaemon carinicauda named EcRelish was 2141 bp encoding a 660 amino-acid polypeptide. EcRelish cDNA contained a conserved Rel homology domain and two nucleus localization signals. Sequence analysis indicated that the deduced amino acid sequence of the EcRelish showed high similarities to that of other crustaceans. Real time RT-PCR analysis showed that EcRelish mRNA expressed with different levels in tested tissues, and the highest expression was observed in the hemocytes. With longer infection time, the cumulative mortality rates increased gradually followed by the proliferation of Vibrio anguillarum and WSSV. The expression profiles of EcRelish gene were analyzed after V. a nguillarum, white spot syndrome virus (WSSV) challenge, and ammonia-N stress. The results showed that the expression levels of EcRelish mRNA in the hemocytes were up-regulated at 1–24 h after V. a nguillarum challenge. Meanwhile, the expression levels of EcRelish mRNA were up-regulated at 3 h after WSSV challenge. The expression of EcRelish in hemocytes was down-regulated significantly under ammonia-N stress during the experimental time. The results indicated that EcRelish might be involved in immune defense against pathogens and ammonia-N stress in E. c arinicauda.

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Acknowledgments

The authors are grateful to all the laboratory members for experimental material preparation and technical assistance. This study was supported by the earmarked fund for National “863” Project of China (No. 2012AA10A409), Modern Agro-industry Technology Research System (No. CARS-47), and the Special Fund for Agro-scientific Research in the Public Interest (No. 201103034).

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Authors and Affiliations

  1. Fisheries College, Ocean University of China, Qingdao, 266003, People’s Republic of China

    Qianqian Ge

  2. Key Laboratory for Sustainable Utilization of Marine Fisheries Resources, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, 266071, People’s Republic of China

    Qianqian Ge, Jitao Li, Jian Li, Fazhen Zhao & Hai Ren

  3. College of Fisheries, Henan Normal University, Xinxiang, 453007, People’s Republic of China

    Junping Liang

  4. Key Laboratory of South China Sea Fishery Resources Exploitation and Utilization, Ministry of Agriculture, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, 510300, People’s Republic of China

    Yafei Duan

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  1. Qianqian Ge
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Ge, Q., Liang, J., Li, J. et al. Molecular cloning and expression analysis of Relish gene from the ridgetail white prawn Exopalaemon carinicauda . Fish Sci 81, 699–711 (2015). https://doi.org/10.1007/s12562-015-0898-z

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