Abstract
The trace element contents of Corbicula clam shells collected from Japan, Russia, China, and the Republic of Korea were analyzed to determine their geographic origin. The crushed shells were decomposed with nitric acid–hydrogen peroxide, and the concentrations of 14 elements (Li, Mg, V, Mn, Co, As, Rb, Mo, Ba, Ce, Pb, U, Sr, and Ca) were measured by inductively coupled plasma mass spectrometry and inductively coupled plasma optical emission spectrometry. Some of the elements identified in samples displayed a geographic trend. The average content of manganese in Japanese samples was twice that of Russian samples. Conversely, the arsenic content in Japanese samples was approximately half of that in Russian samples. Linear discriminant analysis was applied to the data from Japanese and Russian samples, and a discriminant model was constructed. The discriminant model was used to determine the geographic origin of Corbicula clams produced in Japan, with 89.8 % of those identified as Japanese and 92.2 % of those identified as Russian being classified correctly. Therefore, trace element analysis of the shells of Corbicula clams is a useful technique for the identification of their country of origin.
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Acknowledgments
We thank Dr. Mikio Nakamura, Dr. Chika Oshiumi, and the staff of the Japan Corbicula Research Institute for their cooperation with sampling. This study was supported by a project grant (Development of Evaluation and Management Methods for Supply of Safe, Reliable and Functional Food and Farm Produce) from the National Food Research Institute and the Ministry of Agriculture, Forestry and Fisheries of Japan.
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Iguchi, J., Isshiki, M., Takashima, Y. et al. Identifying the origin of Corbicula clams using trace element analysis . Fish Sci 80, 1089–1096 (2014). https://doi.org/10.1007/s12562-014-0775-1
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DOI: https://doi.org/10.1007/s12562-014-0775-1