Abstract
Anguilla luzonensis and A. huangi were each described in 2009 using eels obtained from northern Luzon Island. We examined the taxonomic status of these two groups of eels using morphological and molecular genetic characters. There were no significant differences in two vertebrae counts between eels of A. luzonensis and A. huangi. Mitochondrial 16S ribosomal RNA and cytochrome b genes sequences were obtained and compared among 28 specimens of A. luzonensis, the holotypes of A. luzonensis and A. huangi, and one specimen of the other 15 anguillid species. The specimens of A. luzonensis exhibited almost identical sequences, including the holotype, with only a few site differences, and the genetic difference between the holotypes of A. luzonensis and A. huangi was within the range of differences of specimens of A. luzonensis. The other anguillid species were genetically very different from A. luzonensis and A. huangi, although A. interioris is a closely related species. It is clear that A. luzonensis and A. huangi are the same species, and according to the principle of priority in zoological nomenclature, A. luzonensis Watanabe, Aoyama, and Tsukamoto, 2009 is the valid species name, and A. huangi Teng, Lin, and Tzeng, 2009 is a junior synonym of A. luzonensis.
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References
Ege V (1939) A revision of the genus Anguilla Shaw, a systematic, phylogenetic and geographical study. Dana Rep 16:1–256
Castle PHJ, Williamson GR (1974) On the validity of the freshwater eel species Anguilla ancestralis Ege from Celebes. Copeia 1974:569–570
Watanabe S (2003) Taxonomy of the freshwater eels, genus Anguilla Schrank, 1798. In: Aida K, Tsukamoto K, Yamauchi K (eds) Eel biology. Springer, Tokyo, pp 3–18
Watanabe S, Aoyama J, Tsukamoto K (2009) A new species of freshwater eel Anguilla luzonensis (Teleostei: Anguillidae) from Luzon Island of the Philippines. Fish Sci 75:387–392
Teng H-Y, Lin Y-S, Tzeng C-S (2009) A new Anguilla species and reanalysis of the phylogeny of freshwater eels. Zool Stud 48:808–822
Watanabe S, Aoyama J, Tsukamoto K (2004) Reexamination of Ege’s (1939) use of taxonomic characters of the genus Anguilla. Bull Mar Sci 74:337–351
Aoyama J, Wouthuyzen S, Miller MJ, Minegishi Y, Kuroki M, Suharti SR, Kawakami T, Sumardiharga KO, Tsukamoto K (2007) Distribution of leptocephali of the freshwater eels, genus Anguilla, in the waters off west Sumatra in the Indian Ocean. Environ Biol Fish 80:445–452
Kuroki M, Aoyama J, Wouthuyzen S, Sumardhiharga KO, Miller MJ, Minagawa G, Tsukamoto K (2006) Age and growth of Anguilla interioris leptocephali collected in Indonesian waters. Coastal Mar Sci 30:264–268
Wouthuyzen S, Aoyama J, Sugeha YH, Miller MJ, Kuroki M, Minegishi Y, Suharti S, Tsukamoto K (2009) Seasonality of spawning by tropical anguillid eels around Sulawesi Island, Indonesia. Naturwissenshaften 96:153–158
Aoyama J, Watanabe S, Ishikawa S, Nishida M, Tsukamoto K (2000) Are morphological characters distinctive enough to discriminate between two species of freshwater eels, Anguilla celebesensis and A. interioris? Ichthyol Res 47:157–161
Minegishi Y, Aoyama J, Inoue JG, Azanza RV, Tsukamoto K (2009) Inter- specific and subspecific genetic divergences of freshwater eels, genus Anguilla including a recently described species, A. luzonensis, based on whole mitochondrial genome sequences. Coastal Mar Sci 33:64–77
Aoyama J, Nishida N, Tsukamoto K (2001) Molecular phylogeny and evolution of the freshwater eel, genus Anguilla. Mol Phylogenet Evol 20:450–459
Lin YS, Poh YP, Tzeng CS (2001) A phylogeny of freshwater eels inferred from mitochondrial genes. Mol Phylogenet Evol 20:252–261
Inoue JG, Miya M, Tsukamoto K, Nishida M (2001) Complete mitochondrial DNA sequence of the Japanese eel, Anguilla japonica. Fish Sci 67:118–125
Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG (1997) The CLUSTAL X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882
Kimura M (1980) A simple method for estimating evolutionary rate of base substitution through comparative studies of nucleotide sequences. J Mol Evol 16:111–120
International Commission on Zoological Nomenclature (ICZN) (1999) International code of zoological nomenclature, 4th edn. The International Trust For Zoological Nomenclature, c/o The Natural History Museum, London, xxix + 306 pp
Ellis T, Howell BR, Hayes J (1997) Morphological differences between wild and hatchery-reared turbot. J Fish Biol 50:1124–1128
Kuroki M, Aoyama J, Miller MJ, Wouthuyzen S, Arai T, Tsukamoto K (2006) Contrasting patterns of growth and migration of tropical anguillid leptocephali in the western Pacific and Indonesian Seas. Mar Ecol Prog Ser 309:233–246
Kuroki M, Aoyama J, Miller MJ, Watanabe S, Shinoda A, Jellyman DJ, Feunteun E, Tsukamoto K (2008) Distribution and early life history characteristics of anguillid leptocephali in the western South Pacific. Mar Freshwater Res 59:1035–1047
Tsukamoto K (2006) Spawning of eels near a seamount. Nature 439:929
Tsukamoto K, Chow S, Otake T, Kurogi H, Mochioka N, Miller MJ, Aoyama J, Kimura S, Watanabe S, Yoshinaga T, Shinoda A, Kuroki M, Oya M, Watanabe T, Hata K, Ijiri S, Kazeto Y, Nomura K, Tanaka H (2011) Oceanic spawning ecology of freshwater eels in the western North Pacific. Nat Comm. doi:10.1038/ncomms1174
Aoyama J, Watanabe S, Nishida M, Tsukamoto K (2000) Discrimination of catadromous eels of genus Anguilla using polymerase chain reaction-restriction fragment length polymorphism analysis of the mitochondrial 16S ribosomal RNA domain. Trans Am Fish Soc 129:873–878
Watanabe S, Aoyama J, Nishida M, Tsukamoto K (2005) A molecular genetic evaluation of the taxonomy of eels of the genus Anguilla (Pisces: Anguilliformes). Bull Mar Sci 76:675–690
Aoyama J (2009) Life history and evolution of migration in catadromous eels (genus Anguilla). Aqua-BioSci Monogr 2:1–42
Kuroki M, Miller MJ, Aoyama J, Watanabe S, Yoshinaga T, Tsukamoto K (2012) Evidence of offshore spawning for the newly discovered anguillid species Anguilla luzonensis (Teleostei: Anguillidae) in the western North Pacific. Pac Sci 66:497–508
Acknowledgments
This study could not have been carried out without the kind help of the local fishermen and people who are each independently permitted by federal, regional and local authorities in the Philippines. The authors sincerely thank the following colleagues: T. Ishikawa, T. Umehara, N. Nanato and J. Abadu for their contributions to this research effort, M. Iida for assisting with molecular genetic experiments and M. J. Miller for helping to improve the paper. We also thank M. Oya for her support in many aspects of the project. S. W. was partly supported by the Ito Foundation for Promotion of Ichthyology, a Sasakawa Scientific Research Grant from the Japan Science Society, and Research Fellowships for Young Scientists (No. 18780143) from the Japan Society for the Promotion of Science. This study was supported partly by Grants-in-aid (Nos. 07306022, 08041139, 11691177 12NP0201 and 23580246) from the Ministry of Education, Culture, Sports, Science and Technology of Japan, “Research for the Future” Program No. JSPS–RFTF 97L00901 from the Japan Society for the Promotion of Science, Touwa Shokuhin Shinkoukai, and the Eel Research Foundation of Nobori-kai.
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Watanabe, S., Aoyama, J., Hagihara, S. et al. Anguilla huangi Teng, Lin, and Tzeng, 2009, is a junior synonym of Anguilla luzonensis Watanabe, Aoyama, and Tsukamoto, 2009. Fish Sci 79, 375–383 (2013). https://doi.org/10.1007/s12562-013-0620-y
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DOI: https://doi.org/10.1007/s12562-013-0620-y