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Mitochondrial DNA barcoding for Okinawan oysters: a cryptic population of the Portuguese oyster Crassostrea angulata in Japanese waters

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Abstract

We used mitochondrial (mt) DNA barcoding to evaluate the species diversity of oysters sampled from the coast of Okinawa Island, Japan. Our analysis revealed the presence of a hitherto-unrecognized population of the Portuguese oyster Crassostrea angulata, a putative species closely related to the Pacific oyster C. gigas. Forty-eight Okinawan oyster specimens were collected and subjected to species diagnosis trials based on mtDNA barcodes. Of the 30 Crassostrea specimens, 17 were identified as C. angulata, seven as C. gigas and six as C. bilineata (syn. C. iredalei). The remaining 18 Saccostrea specimens formed several divergent clades in phylogenetic trees, although they could not be identified to species level because of the confounding taxonomy of the Saccostrea species complex. Our results stress the necessity for reviewing the species diversity of Okinawan oysters.

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Acknowledgments

We acknowledge the generosity of Dr. Tomoyuki Nakano, Field Science Education and Research Center of Kyoto University, who kindly provided us some of the articles cited in our study. We thank two anonymous reviewers and the handling editor for their comments on the manuscript. Financial support for this study was provided in part by a grant-in-aid (to HY) from Japan Society for the Promotion of Science (KAKENHI No. 23401005).

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  1. Research Center for Aquatic Genomics, National Research Institute of Fisheries Science, Fisheries Research Agency, 2-12-4 Fukuura, Kanazawa, Yokohama, Kanagawa , 2368648, Japan

    Masashi Sekino

  2. Malaco-diversity Japan, 3-1-26-103 Matsugaoka, Kugenuma, Fujisawa, Kanagawa, 2510038, Japan

    Hiroyoshi Yamashita

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  1. Masashi Sekino
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  2. Hiroyoshi Yamashita
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Correspondence to Masashi Sekino.

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Sekino, M., Yamashita, H. Mitochondrial DNA barcoding for Okinawan oysters: a cryptic population of the Portuguese oyster Crassostrea angulata in Japanese waters. Fish Sci 79, 61–76 (2013). https://doi.org/10.1007/s12562-012-0577-2

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