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Assessment and Evaluation of an Integrated Hybrid Anaerobic–Aerobic Sewage Treatment System for the Removal of Enteric Viruses

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Abstract

The capability of a cost-effective and a small size decentralized pilot wastewater treatment plant (WWTP) to remove enteric viruses such as rotavirus, norovirus genogroup I (GGI), norovirus genogroup II (GGII), Hepatitis E virus (HEV), and adenovirus was studied. This pilot plant is an integrated hybrid anaerobic/aerobic setup which consisted of anaerobic sludge blanket (UASB), biological aerated filter (BAF), and inclined plate settler (IPS). Both the UASB and BAF are packed with a non-woven polyester fabric (NWPF). Results indicated that the overall log10 reductions of enteric viruses’ genome copies through the whole system were 3.1 ± 1, 3.3 ± 0.5, and 2.6 ± 0.9 log10 for rotavirus, norovirus GGI, and adenovirus, respectively. Reduction efficiency for both norovirus GGII and HEV after the different treatment steps could not be calculated because there were no significant numbers of positive samples for both viruses. The overall reduction of rotavirus infectious units through the whole system was 2.2 ± 0.8 log10 reduction which is very close to the overall log10 reduction of adenovirus infectious units through the whole system which was 2.1 ± 0.8 log10 reduction. There was no considerable difference in the removal efficiency for different rotavirus G and P types. Adenovirus 41 was the only type detected in the all positive samples. Although the pilot WWTP investigated is cost effective, has a small footprint, does not need a long distance network pipes, and easy to operate, its efficiency to remove enteric viruses is comparable with the conventional centralized WWTPs.

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Acknowledgement

The authors would like to thank the Egyptian Academy of Scientific Research and Technology (ASRT), the Science and Technology Development fund (STDF) for basic, and research Grant No. 1088.

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  1. Environmental Virology Lab., Department of Water Pollution Research, National Research Centre (NRC), 33 El-Buhouth st., Dokki, Giza, P.O. 12622, Egypt

    Waled Morsy El-Senousy

  2. Wastewater Treatment Lab., Department of Water Pollution Research, National Research Centre (NRC), 33 El-Buhouth st., Dokki, Giza, P.O. 12622, Egypt

    Sohair Imam Abou-Elela

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  1. Waled Morsy El-Senousy
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El-Senousy, W.M., Abou-Elela, S.I. Assessment and Evaluation of an Integrated Hybrid Anaerobic–Aerobic Sewage Treatment System for the Removal of Enteric Viruses. Food Environ Virol 9, 287–303 (2017). https://doi.org/10.1007/s12560-017-9286-4

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