Abstract
The study was designed to investigate the protective effect of esculin against pro-oxidant aflatoxin B1 (AFB1)-induced nephrotoxicity in mice. In this study toxicity was developed by oral administration of AFB1 at a dose of 66.60 μg/kg bw/day for 90 days in male Swiss albino mice. Esculin (150 mg/kg bw/0.2 ml/day) and standard compound ascorbic acid (300 mg/kg bw/0.2 ml/day) was given after 30 min of AFB1 administration for 90 days. Protective efficacy was assessed by measuring the levels of lipid peroxidation (LPO) and non-enzymatic antioxidants such as reduced glutathione (GSH) and also by measuring activities of enzymatic antioxidants such as glutathione peroxidase (GPX), glutathione-S-transferase (GST), glutathione reductase (GR), superoxide dismutase (SOD) and catalase (CAT) in kidney. Results were analysed at the 30th, 60th and 90th day of the daily treatments, which showed a decrease in the level of LPO and an increase in the levels of enzymatic and non-enzymatic antioxidants. The protective effect of esculin was further proved by histopathological findings as it exhibited regenerative activities in mice renal tubules against AFB1-induced nephrotoxicity. The results obtained clearly demonstrate that the protective efficacy of esculin against pro-oxidant AFB1-induced nephrotoxicity in mice might be due to its antioxidants and free radical scavenging properties.
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Abbreviations
- AFB1 :
-
aflatoxin B1
- Asc:
-
ascorbic acid
- bw:
-
body weight
- CAT:
-
catalase
- CDNB:
-
1- Chloro-2, 4-Dinitro Benzene
- DDW:
-
double-distilled water
- DTNB:
-
5,5′-dithiobis(2-nitro-benzoic acid)
- EDTA:
-
ethelene diamine tetra acetic acid
- GPX:
-
glutathione peroxidase
- GR:
-
glutathione reductase
- GSH:
-
reduced glutathione
- GSSG:
-
oxidised glutathione
- GST:
-
glutathione-S-tranferase 2
- JHAEC:
-
Jamia Hamdard Animal Ethics Committee
- LPO:
-
lipid peroxidation
- NADPH:
-
nicotinamide adenine dinucleotide phosphates
- NBT:
-
nitro-blue tetrazolium salt
- PBS:
-
phosphate buffered saline
- PMS:
-
post-mitochondrial supernatant
- ROS:
-
reactive oxygen species
- SOD:
-
superoxide dismutase
- TBARS:
-
thio-barbituric acid reactive substances
- TBA:
-
thio-barbituric acid
- TCA:
-
tri-chloro acetic acid
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The authors are thankful to TMOP & M/CSIR, UGC-SAP, DST and ICMR Govt. of India for financial support and infrastructure facilities to carry out this study.
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Naaz, F., Abdin, M.Z. & Javed, S. Protective effect of esculin against prooxidant aflatoxin B1-induced nephrotoxicity in mice. Mycotoxin Res 30, 25–32 (2014). https://doi.org/10.1007/s12550-013-0185-8
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DOI: https://doi.org/10.1007/s12550-013-0185-8