Mycotoxin Research

, Volume 26, Issue 4, pp 217–220

Blood plasma levels of deoxynivalenol and its de-epoxy metabolite in broilers after a single oral dose of the toxin

  • Agha Waqar Yunus
  • Hana Valenta
  • Sherif M. Abdel-Raheem
  • Susanne Döll
  • Sven Dänicke
  • Josef Böhm
Original Paper

DOI: 10.1007/s12550-010-0057-4

Cite this article as:
Yunus, A.W., Valenta, H., Abdel-Raheem, S.M. et al. Mycotox Res (2010) 26: 217. doi:10.1007/s12550-010-0057-4

Abstract

To evaluate the transfer of deoxynivalenol (DON) and its de-epoxy metabolite (de-epoxy-DON) in the plasma of chicken, mashed oats naturally contaminated with 9.5 mg DON/kg were fed to four broilers (35 days age) at a dose of 20 g/bird. Blood samples were then collected from two birds at 1 h, 3 h, and 5 h post-feeding, while from the other two birds at 2 h, 4 h, and 6 h post-feeding. Analysis of DON and de-epoxy-DON was carried out by using liquid chromatography-tandem mass spectrometry after clean-up with immunoaffinity columns. At 1 h, 3 h, and 5 h post-feeding, the average values of plasma DON were 0.35 ng/ml, 0.20 ng/ml, and 0.15 ng/ml, respectively. The corresponding average values of de-epoxy-DON at these time points were 0.70 ng/ml, 0.80 ng/ml, and 0.25 ng/ml, respectively. The sum of DON and de-epoxy-DON appearing in the plasma at 1 h post-feeding in these birds was estimated to be 0.044% of the total DON fed. At 2 h, 4 h, and 6 h post-feeding, the average values of plasma DON were 0.85 ng/ml, 0.45 ng/ml, and 0.30 ng/ml. De-epoxy-DON could not be detected in the birds sampled at 2 h, 4 h, and 6 h post-feeding. The total amount of DON appearing in the plasma at 2 h post-feeding in these birds was estimated to be 0.036% of the DON fed. These data show that the absorption rate of DON is very low in broilers and that there is also a rapid transformation, and clearance from plasma. Furthermore, there appeared to be individual variability in the capacity of birds to de-epoxidise DON.

Keywords

DeoxynivalenolDe-epoxy-deoxynivalenolPlasmaBroiler

Copyright information

© Society for Mycotoxin Research and Springer 2010

Authors and Affiliations

  • Agha Waqar Yunus
    • 1
  • Hana Valenta
    • 2
  • Sherif M. Abdel-Raheem
    • 3
  • Susanne Döll
    • 2
  • Sven Dänicke
    • 2
  • Josef Böhm
    • 1
  1. 1.Institute of Animal NutritionUniversity of Veterinary Medicine ViennaViennaAustria
  2. 2.Institute of Animal NutritionFriedrich-Loeffler-InstituteBraunschweigGermany
  3. 3.Nutrition and Clinical Nutrition Department, Faculty of Veterinary MedicineAssiut UniversityAssiutEgypt