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Change in Glutenin Macropolymer Secondary Structure in Wheat Sourdough Fermentation by FTIR

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Abstract

Wheat sourdough was prepared by fermentation with Lactobacillus plantarum M616 and yeast in the present study. The change in secondary structure of glutenin macropolymer (GMP) in wheat sourdough fermentation for 4 and 12 h was determined using Fourier transform infrared spectroscopy, and then the resultant spectra were Fourier self-deconvoluted of the amide I band in the region from 1600 to 1700 cm−1. Significant different spectra especially in the amide I band for GMP from sourdough fermented with L. plantarum M616 (SL) and with L. plantarum M616 and yeast (SLY) were found in respect of control dough (CK), dough with acids (SA), and sourdough fermented with yeast (SY) at 4 and 12 h of fermentation. The loss of α-helix structure in SL, SLY, and SA samples was noticed during fermentation. Compared with CK and SY, SL, SLY, and SA samples showed significant decrease (p < 0.05) in the relative areas of α-helix at the same stage of fermentation. In addition, β-turns in SL sourdough decrease, and the relative areas of random coil increase significantly (p < 0.05). These changes in the secondary structure mean that the flexibility of glutenin macropolymer in sourdough increases and it makes GMP degradation easier during fermentation. The modified secondary structure of GMP makes more sensitive to proteolysis by means of cereal enzymes.

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Acknowledgements

The authors thanks for the financial support of National Key Research and Development Program (2016YFD0400200), National Natural Science Foundation of China (31571780), and Natural Science Project of Henan Science and Technology Department (162102210193).

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Authors and Affiliations

  1. College of Biological Engineering, Henan University of Technology, Zhengzhou, 450001, China

    Jinshui Wang, Yuanyuan Yue, Tiantian Liu, Bin Zhang, Zhenlei Wang & Changfu Zhang

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  1. Jinshui Wang
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  2. Yuanyuan Yue
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  4. Bin Zhang
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  5. Zhenlei Wang
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Correspondence to Jinshui Wang.

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Wang, J., Yue, Y., Liu, T. et al. Change in Glutenin Macropolymer Secondary Structure in Wheat Sourdough Fermentation by FTIR. Interdiscip Sci Comput Life Sci 9, 247–253 (2017). https://doi.org/10.1007/s12539-016-0206-3

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