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Epigenetic floral homeotic mutation in pD991-AP3-derived T-DNA-tagged lines for CTP:Phosphorylcholine cytidylyltransferase (CCT) Genes: The homeotic mutation of the cct1-1 allele is enhanced by the cct2 allele and alleviated by CCT1 overexpression

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Abstract

The apetala3 (ap3)-like homeotic mutation (ap3-HM) is recognized among pD991-AP3-derived Arabidopsis thaliana T-DNA-tagged lines carrying the -448 to +47 region of AP3 in their T-DNA. In the corresponding mutant lines for CTP:phosphorylcholine cytidylyltransferase genes, cct1-1 and cct2 (Inatsugi et al. 2009), some flowers of cct1-1 (F4) and many flowers of cct1-1 cct2 (F3) showed ap3-HM, and all flowers of cct1-1 (F5) and cct1-1 cct2 (F4) became increasingly homeotic. In contrast, cct2 flowers were normal for all generations tested. These results demonstrated that ap3-HM is linked to the cct1-1 allele and is enhanced by the cct2 allele. The ap3-HM in cct mutants was inversely correlated with AP3 transcript levels in enriched flower buds. Bisulfite sequencing revealed severe methylation within endogenous AP3 promoter regions in cct1-1 (F3; -317 to -2) and cct1-1 cct2 (F3; -473 to -2), but wild-type (Wassilevskaja) and cct2 plants showed no corresponding methylation. The ap3-HM in cct1-1 cct2 mutants was fully rescued by expressing a PISTILLATA promoter–AP3 construct, and was better alleviated in the F1 offspring of a cross with the CCT1-overexpressing mutant cct1-2 (Columbia) than with the wild type. We discuss possible links between expression of CCT and suppression of ap3-HM.

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Abbreviations

AP3 :

APETALA3

CCT, CTP:

phosphorylcholine cytidylyltransferase

Col:

Columbia

GFP:

green fluorescent protein

HDGS:

homologydependent gene silencing

PI :

PISTILLATA

ProAP3 :

AP3 promoter

ProPI :

PI promoter

Pro35S :

Cauliflower mosaic virus 35S promoter

siRNA:

small interfering RNA

WS:

Wassilevskaja

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Author notes

  1. Yoshinori Hayakawa

    Present address: Theoretical Biology Laboratory, RIKEN, Wako, 351-0198, Japan

  2. Yasuyo Yamaoka

    Present address: Department of Integrative Bioscience and Biotechnology, World Class University Program, Pohang University of Science and Technology, Pohang, 790-784, Korea

  3. Rie Inatsugi

    Present address: Institute of Evolutionary Biology and Environmental Studies, University of Zurich, Winterthurerstrasse 190, 8057, Zurich, Switzerland

Authors and Affiliations

  1. Laboratory of Plant Molecular Physiology, Division of Life Science, Graduate School of Science and Engineering, Saitama University, Shimo-Okubo 255, Sakura-Ku, Saitama, 338-8570, Japan

    Yoshinori Hayakawa, Zhongrui Duan, Miki Yadake, Jun Tsukano, Yasuyo Yamaoka, Yuki Fujiki & Ikuo Nishida

  2. Departmenet of Biological Sciences, Graduate School of Science, The University of Tokyo, Hongo 7-3-1, Bunkyo-ku, Tokyo, 113-0033, Japan

    Rie Inatsugi

  3. Center for Sustainable Resource Science, RIKEN, Yokohama, Kanagawa, Japan

    Akira Oikawa & Kazuki Saito

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  1. Yoshinori Hayakawa
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Correspondence to Ikuo Nishida.

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Hayakawa, Y., Duan, Z., Yadake, M. et al. Epigenetic floral homeotic mutation in pD991-AP3-derived T-DNA-tagged lines for CTP:Phosphorylcholine cytidylyltransferase (CCT) Genes: The homeotic mutation of the cct1-1 allele is enhanced by the cct2 allele and alleviated by CCT1 overexpression. J. Plant Biol. 58, 183–192 (2015). https://doi.org/10.1007/s12374-014-0587-y

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