Abstract
The fungus Chaetomium gracile is used for production of dextranase and removal of dextran during sugar manufacturing process, however, the productivity of dextranase is low. In this study, we investigated the influence of carbon source on dextranase production using dextrans of different sizes. Culturing C. gracile in medium containing crude dextran and use of high molecular weight of dextrans resulted in the higher yield of dextranase production (80.5 ± 4.0 U ml−1). Cells incubated in medium containing glucose as sole carbon source exhibited a high growth rate but did not produce dextranase. Based on these findings, fed-batch and two-step fermentation strategies were employed to further increase the yield of dextranase with production of 159.5 and 187.0 U ml−1, respectively.
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Acknowledgments
This work was supported by the National Natural Science Foundation of China (31460026, 31560027), the Key Fundamental Research Fund of the Education Department of Guangxi Province (ZD2014001), the Key Scientific Research Fund of Guangxi Province (14122004-2), and the Fundamental Research Fund of Guangxi University (XJZ140293).
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Li, K., Lu, H., Hang, F. et al. Improved Dextranase Production by Chaetomium gracile Through Optimization of Carbon Source and Fermentation Parameters. Sugar Tech 19, 432–437 (2017). https://doi.org/10.1007/s12355-016-0476-4
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DOI: https://doi.org/10.1007/s12355-016-0476-4