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Phenolics Content and Inhibitory Effect of Sugarcane Molasses on α-Glucosidase and α-Amylase In Vitro

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Abstract

Sugarcane molasses is a wealthy source of health beneficial phenolic compounds and exhibited significant biological properties. Present study was designed to assess the inhibitory effect of sugarcane molasses on α-glucosidase and α-amylase. Hypoglycemic fractions were extracted using macro-porous and ion exchange resins. The total phenolics content was 0.179 ± 0.003 mg GAE per milligram of extract. Four phenolic acids which include caffeic acid (11.64 mg/g), ferulic acid (10.49 mg/g), chlorogenic acid (1.77 mg/g), and gallic acid (0.87 mg/g) were identified and quantified by high-performance liquid chromatography. The inhibitory activities of sugarcane molasses were 4.693 mg/mL (Km = 1.099 mL/mg) and 4.254 mg/mL (Km 0.238 mL/mg) for α-glucosidase and α-amylase, respectively, which revealed that sugarcane molasses could be a useful addition in medicinal preparations as nutraceutical and functional food for diabetic patients.

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Acknowledgments

This work was supported by the ministry of science and technology in agriculture science and technology Achievements Transformation Fund Project (No. 2013GB23600669), the Science and Technology Planning Project of Guangdong Province, China (No. 2011B050400035), the Science and Technology Planning Project of Guang Zhou, China (No. 2013J4500036) and the Open Project Program of Provincial Key Laboratory of Green Processing Technology and Product Safety of Natural Products (201303).

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Authors and Affiliations

  1. College of Light Industry and Food Sciences, South China University of Technology, Guangzhou, 510640, People’s Republic of China

    Fansheng Kong, Shujuan Yu, Feng Zeng & Xinlan Wu

  2. College of Pharmacy, Guangdong Pharmaceutical University, Guangzhou, People’s Republic of China

    Fansheng Kong

  3. Guangdong Province Key Laboratory for Green Processing of Natural Products and Product Safety, Guangzhou, 510640, People’s Republic of China

    Fansheng Kong & Shujuan Yu

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  1. Fansheng Kong
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Correspondence to Shujuan Yu.

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Kong, F., Yu, S., Zeng, F. et al. Phenolics Content and Inhibitory Effect of Sugarcane Molasses on α-Glucosidase and α-Amylase In Vitro. Sugar Tech 18, 333–339 (2016). https://doi.org/10.1007/s12355-015-0385-y

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