Skip to main content
Log in

Genetic and Symptomatic Characterization of Sugarcane mosaic virus (SCMV) in Cuba

  • Research Article
  • Published:
Sugar Tech Aims and scope Submit manuscript

Abstract

Sugarcane mosaic resistance is established as a key criterion for the breeding program in Cuba. However, molecular characterization of Sugarcane mosaic virus (SCMV) strains occurring in Cuba has not been reported so far. Leaf samples from commercial sugarcane cultivars were collected for SCMV screening in three testing sites (the Matanzas, Camagüey and Holguin provinces). Reverse-Transcriptase polymerase chain reaction was used to identify the virus. The nucleotide sequence comparison grouped the isolates into SCMV subgroups. Sugarcane cultivars inoculated with SCMV from three testing sites displayed different symptoms. Results should be decisive to generate the highest selection pressure, increasing the efficiency of the sugarcane breeding program to the SCMV resistance in the commercial cultivars.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

References

  • Acevedo, R., B. Díaz, D. Piñón, E. Fernández, and M. Fajardo. 1993. Behavior of some physiologic indicators in sugarcane infected with Sugarcane mosaic virus (SCMV). Revista de Protección Vegetal 8: 291–299.

    Google Scholar 

  • Adams, M.J., J.F. Antoniw, and C.M. Fauquet. 2005. Molecular criteria for genus and species discrimination within the family Potyviridae. Archives of Virology 150: 459–479.

    Article  CAS  PubMed  Google Scholar 

  • Alegria, O.M., M. Royer, M. Bousalem, M. Chatenet, M. Peterschmitt, J.C. Girard, and P. Rott. 2003. Genetic diversity in the coat protein coding region of eighty-six Sugarcane mosaic virus isolates from eight countries, particularly from Cameroon and Congo. Archives of Virology 148: 357–372.

    Article  CAS  PubMed  Google Scholar 

  • Annicchiarico, P. 2002. Genotype x environment interactions. Challenges and opportunities for plant breeding and cultivar recommendations. FAO Plant Production and Protection 174: 2–4.

    Google Scholar 

  • Brunt, A.A. 1992. The general properties of Potyviruses. Archives of Virology 5: 3–16.

    Article  CAS  PubMed  Google Scholar 

  • Chare, E.R., and E.C. Holmes. 2006. A phylogenetic survey of recombination frequency of plant RNA virus. Archives of Virology 151: 933–946.

    Article  CAS  PubMed  Google Scholar 

  • Chen, J., J. Chen, and M.J. Adams. 2002. Molecular characterization of potyviruses from sugarcane and maize in China. Archives of Virology 147: 1237–1246.

    Article  CAS  PubMed  Google Scholar 

  • Espejel, F., D. Jeffers, J.C. Noa-Carrazana, S. Ruiz-Castro, and L. Silva-Rosales. 2006. Coat protein gene sequence of a Mexican isolate of Sugarcane mosaic virus and its infectivity in maize and sugarcane plants. Archives of Virology 151: 409–412.

    Article  CAS  PubMed  Google Scholar 

  • Gaur, R.K., M. Singh, and G.P. Rao. 2003. Molecular Characterization of Sugarcane Mosaic Virus isolate from north eastern region of India. Sugarcane 5: 149–153.

    CAS  Google Scholar 

  • Goodman B S. (1999) A study of South African strains of Sugarcane Mosaic Potyvirus (SCMV) identified by sequence analysis of the 5´region of the coat protein gene. MS thesis, Department of Biotechnology, Durban University of Technology, Durban, South Africa.

  • Grisham, M.P., and Y.B. Pan. 2007. A genetic shift in the virus strains that cause mosaic in louisiana sugarcane. Plant Disease 91: 453–458.

    Article  CAS  Google Scholar 

  • Hall, J.S., B. Adams, T.J. Parsons, R. French, L.C. Lane, and S.G. Jensen. 1998. Molecular cloning, sequencing and phylogenetic relationship of a new potyvirus: Sugarcane Streak Mosaic Virus, and a reevaluation of the classification of the Potyviridae. Molecular Biology and Evolution 10: 323–332.

    CAS  Google Scholar 

  • Handley, J.A., G.R. Smith, J.L. Dale, and R.M. Harding. 1998. Sequence diversity in the coat protein coding region of twelve Sugarcane mosaic potyvirus isolates from Australia, USA and South Africa. Archives of Virology 143: 1145–1153.

    Article  CAS  PubMed  Google Scholar 

  • INICA. (2011) Manual de Normas y Procedimientos del Programa de Fitomejoramiento. Boletín Especial Cuba & Caña, p. 200.

  • Jiang, J.X., X.P. Zhou, and Y.L. Shi. 2002. Identification of the viral pathogen of sugarcane mosaic disease in Hangzhou. Journal of Agricultural Biotechnology 10: 377–380.

    Google Scholar 

  • Koike, H., and A.G. Gillaspie Jr. 1989. Mosaic. In Diseases of sugarcane. Major diseases, ed. C. Ricaud, B.T. Egan, A.G. Gillaspie Jr, and C.G. Hughes, 400 . Amsterdam: Elsevier.

    Google Scholar 

  • Kong, P., and H.H. Steinbiss. 1998. Complete nucleotide sequence and analysis of the putative polyprotein of Maize dwarf mosaic virus genomic RNA (Bulgarian isolate). Virology 143: 1791–1799.

    CAS  Google Scholar 

  • Marie-Jeanne, V., R. Ioos, J. Peyre, B. Alliot, and P. Signoret. 2000. Differentiation of Poaceae Potyviruses by reverse transcription polymerase chain reaction and restriction analysis. Journal of Phytopathology 148: 141–151.

    Article  CAS  Google Scholar 

  • Oertel, U., J. Schubert, and E. Fuchs. 1997. Sequence comparison of the 3′-terminal parts of the RNA of four German isolates of Sugarcane mosaic potyvirus (SCMV). Archives of Virology 142: 675–687.

    Article  CAS  PubMed  Google Scholar 

  • Padhi, A., and K. Ramu. 2011. Genomic evidence of intraspecific recombination in Sugarcane mosaic virus. Virus Genes 42: 282–285.

    Article  CAS  PubMed  Google Scholar 

  • Perera, M.F., M.P. Filippone, C.J. Ramallo, M.I. Cuenya, M.L. García, L.D. Ploper, and A.P. Castagnaro. 2009. Genetic diversity among viruses associated with sugarcane mosaic disease in Tucumán, Argentina. Phytopathology 99: 38–49.

    Article  CAS  PubMed  Google Scholar 

  • Putra, L.K., J.O. Helen, P.J. Anthony, and J.L. Whittle. 2003. Distribution of Sugarcane mosaic virus in sugarcane plants. Australasian Plant Pathology 32: 305–307.

    Article  Google Scholar 

  • Shukla, D.D., and C.W. Ward. 1988. Amino acid sequence homology of coat protein as a basis for identification and classification of the potyvirus group. Journal of General Virology 69: 2703–2710.

    Article  CAS  Google Scholar 

  • Thompson, J.D., T.J. Gibson, F. Plewniak, F. Jeanmouging, and D.G. Higgins. 1997. CLUSTAL X windows interface: Flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleid Acids Research 25: 4876–4882.

    Article  CAS  Google Scholar 

  • Viswanathan, R., R. Karuppaiah, and M. Balamuralikrishnan. 2009. Identification of new variants of SCMV causing sugarcane mosaic in India and assessing their genetic diversity in relation to SCMV type strains. Virus Genes 39: 375–386.

    Article  CAS  PubMed  Google Scholar 

  • Xu, D.L., J.W. Park, T.E. Mirkov, and G.H. Zhou. 2008. Viruses causing mosaic disease in sugarcane and their genetic diversity in southern China. Archives of Virology 153: 1031–1039.

    Article  PubMed  Google Scholar 

  • Yang, Z.N., and T.E. Mirkov. 1997. Sequence and relationships of Sugarcane mosaic and Sorghum mosaic virus strains and development of RT-PCR-based RFLPs for strain discrimination. Phytopathology 87: 932–939.

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgments

The work was supported by the Sugarcane Research Institute, Cuba. The authors would like to thank the Pathology Department for their assistance in the sample and data collection, as well the anonymous referees for their useful critical and recommendations.

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Ariel D. Arencibia.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Puchades, Y., La O, M., Montalván, J. et al. Genetic and Symptomatic Characterization of Sugarcane mosaic virus (SCMV) in Cuba. Sugar Tech 18, 184–191 (2016). https://doi.org/10.1007/s12355-015-0375-0

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12355-015-0375-0

Keywords

Navigation