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New Polymorphic EST-SSR Markers in Sugarcane

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Abstract

New polymorphic SSR markers in sugarcane has potential usefulness to studies genetic diversity, genetic mapping, DNA fingerprinting and determination offspring as true hybrids, selfing or contaminant in germplasm from breeding program. SSR markers can be obtained in several ways, and the search for SSR-EST sequences an alternative with significant results. The aim of this study was to develop highly polymorphic SSR markers for germplasm studies in sugarcane. Di- tri- and tetra-nucleotide sequences were mined in the TGI bank and 53 EST-SSR markers developed. These markers were analyzed in five genotypes and showed to be highly polymorphic, with the PIC ranging between 0.48 and 0.95 with a mean of 0.86. The discriminatory power ranged from 0.35 to 0.94 with a mean of 0.82 and the genetic similarity was high in the accessions evaluated.

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Acknowledgments

Banco do Nordeste and FINEP for financing the project. PMGA for support and supplying biological material. UFAL for granting the PIBIC scholarship to students Dennis Crystian Silva, Marislane Souza and Luiz Sérgio Costa Duarte Filho.

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Authors and Affiliations

  1. Laboratory of Genetics Resources, Campus Arapiraca, Federal University of Alagoas, Avenida Manoel Severino Barbosa s/n, Rodovia AL 115, km 6,5. Bairro Bom Sucesso, Arapiraca-AL, Brazil

    Dennis Crystian Silva, Marislane Carvalo Paz de Souza, Luiz Sérgio Costa Duarte Filho & Cícero Almeida

  2. PMGCA/RIDESA/UFAL, Center for Agricultural Sciences, Federal University of Alagoas, Maceió-AL, Brazil

    João Messias dos Santos & Geraldo Veríssimo de Souza Barbosa

Authors
  1. Dennis Crystian Silva
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  2. Marislane Carvalo Paz de Souza
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  3. Luiz Sérgio Costa Duarte Filho
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  4. João Messias dos Santos
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  5. Geraldo Veríssimo de Souza Barbosa
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  6. Cícero Almeida
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Correspondence to Cícero Almeida.

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Silva, D.C., de Souza, M.C.P., Filho, L.S.C.D. et al. New Polymorphic EST-SSR Markers in Sugarcane. Sugar Tech 14, 357–363 (2012). https://doi.org/10.1007/s12355-012-0184-7

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  • DOI: https://doi.org/10.1007/s12355-012-0184-7

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