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An efficient in vitro shoot regeneration from leaf petiolar explants and ex vitro rooting of Bixa orellana L.- A dye yielding plant

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Abstract

Bixa orellana L. (Bixaceae) is a multipurpose tree grown for the production of commercially important dyes. In the present study, an efficient, reproducible protocol was developed for direct plant regeneration from in vitro derived petiole explants of Bixa orellana L. Murashige and Skoog medium (MS) supplemented with 2-isopentenyl adenine (9.8 μM) and naphthalene acetic acid (10.7 μM) was found to be optimum for production of high frequency of shoot organogenesis. Subculturing of the shoots onto the fresh MS medium containing similar concentrations of 2-iP (9.8 μM) and NAA (10.7 μM) produced elongated shoots. Elongated shoots when placed onto MS medium supplemented with 1.7 μM indole-3-acetic acid and 14.7 μM 2-iP produced optimal rooting. Rooted plantlets were acclimatized and transplanted to the field successfully. Histological investigation revealed the origin of shoot primordia, from sub-epidermal cells of petiole explants. The regeneration protocol developed in this study can be useful for mass in vitro propagation and effective genetic transformation of commercially important edible dye yielding tree species.

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Abbreviations

NAA:

α-naphthalene acetic acid

2-iP:

2-isopentenyl adenine

IAA:

Indole-3-acetic acid

BAP:

6-benzylaminopurine

PGR:

Plant growth regulator

WPM:

woody plant medium

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Acknowledgements

We thank Dept. of Botany, SV University,Tirupati for providing us required chemicals and facilities. We highly appreciate our lab members for critical reading of the manuscript. The authors are highly grateful to the UMK for their logistical support under Grant No. R/SGJP/A07.00/00710A/001/2012/000081).

Author contributions

Conceived and designed the experiments: AM,KKC,GRG. Performed the experiments: AM, KKC. Analyzed the data: KKC,AM,GRG. Wrote the paper: KKC, AM,GRG.

Conflict of interest

Authors declare that there is no competing interest.

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Authors and Affiliations

  1. Faculty of Agrobased Industry, Universiti Malaysia Kelantan, Jeli Campus, Locked bag-100, 17600, Jeli, Kelantan, Malaysia

    Arifullah Mohammed

  2. Department of Botany, Division of Plant Tissue Culture, Sri Venkateswara University, Tirupati, Andhra Pradesh, India

    Arifullah Mohammed, Kishore K. Chiruvella & Rama Gopal Ghanta

  3. Department of Molecular Biology and Cell Biology, Tezpur University, Assam, India

    Nima D. Namsa

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  1. Arifullah Mohammed
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  2. Kishore K. Chiruvella
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  4. Rama Gopal Ghanta
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Correspondence to Arifullah Mohammed.

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Mohammed, A., Chiruvella, K.K., Namsa, N.D. et al. An efficient in vitro shoot regeneration from leaf petiolar explants and ex vitro rooting of Bixa orellana L.- A dye yielding plant. Physiol Mol Biol Plants 21, 417–424 (2015). https://doi.org/10.1007/s12298-015-0297-z

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  • DOI: https://doi.org/10.1007/s12298-015-0297-z

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