Physiology and Molecular Biology of Plants

, Volume 19, Issue 2, pp 245–250

RAPD, SCAR and conserved 18S rDNA markers for a red-listed and endemic medicinal plant species, Knema andamanica (Myristicaceae)

Authors

    • Division of Crop Improvement and BiotechnologyIndian Institute of Spices Research
  • P. R. Anju
    • Division of Crop Improvement and BiotechnologyIndian Institute of Spices Research
  • R. S. Shalini
    • Division of Crop Improvement and BiotechnologyIndian Institute of Spices Research
  • S. Siju
    • Division of Crop Improvement and BiotechnologyIndian Institute of Spices Research
  • K. Dhanya
    • Division of Crop Improvement and BiotechnologyIndian Institute of Spices Research
    • Weill Cornell Medical College in Qatar, Qatar Foundation – Education City
  • B. Krishnamoorthy
    • Division of Crop Improvement and BiotechnologyIndian Institute of Spices Research
Research Article

DOI: 10.1007/s12298-013-0166-6

Cite this article as:
Sheeja, T.E., Anju, P.R., Shalini, R.S. et al. Physiol Mol Biol Plants (2013) 19: 245. doi:10.1007/s12298-013-0166-6

Abstract

Knema andamanica is a red-listed endemic medicinal species of Myristicaceae restricted to Andaman and Nicobar (A&N) Islands, India. This species is used in tribal medicines and has immense bioprospective potential. With a view to generate suitable genomic markers for classification and identification, we have generated RAPD, SCAR and conserved 18S rDNA markers from K. andamanica. A unique 585 bp fragment, that distinguished it from seven other related species of Myristicaceae was first amplified using the random primer OPE 06 and converted to SCAR marker (GenBank accession # JN228256). The conserved sequences of 18S rDNA loci from K. andamanica were also amplified and sequenced (GenBank accession #JN228265). The sequence revealed deviations including 18 variable regions and 15 indels that were unique to K. andamanica. These markers can help in definite identification of K. andamanica even at the juvenile stages.

Keywords

Knema andamanicaRAPDSCAR18S rDNA and germplasm characterizationGenetic differentiation

Abbreviations

SCAR

Sequence Characterised Amplified Region

ISSR

Inter Simple Sequence Repeats

AFLP

Amplified Fragment Length Polymorphism

SSR

Simple Sequence Repeats

rDNA

ribosomal DNA

NCBI

National Center for Biotechnology Information

RAPD

Random Amplification of Polymorphic DNA

SNP

Single Nucleotide Polymorphism

BLAST

Basic Local Alignment Search Tool

Copyright information

© Prof. H.S. Srivastava Foundation for Science and Society 2013