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Assessment of FTO Gene Polymorphism and its Association with Type 2 Diabetes Mellitus in North Indian Populations

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Abstract

FTO gene polymorphism related to type 2 diabetes and obesity was studied in this north Indian population. This study was done, due to a continuous increase in the risk of obesity and type 2 diabetes in north Indian population, because of lifestyle and genetic variations. Clinically diagnosed subjects of type 2 diabetes mellitus (as per ADA criteria) were taken as cases and age and sex matched subjects without any associated illness were taken as controls. Obesity was estimated by calculating waist circumference and BMI in the study cases and controls. For genetic variation, DNA was isolated with Quaigen kit method and isolated DNA was amplified with PCR. Amplified DNA was resolved in 1% agarose gel electrophoresis. The Hardy–Weinberg equilibrium, OR, CI and P value were calculated using standard protocols. FTO gene polymorphism (SNP 9940128) was found to be significantly correlated with type 2 diabetes mellitus and obesity. The AG genotype frequency was observed to be higher (13.09%) with (P < 0.0001) in the cases as compared to controls. Logistic regression analysis was conducted for AG and GG genotypes with respect to AA. In this novel study genetic co-relation was observed between FTO gene polymorphisms and type 2 diabetes and obesity in the north Indian population.

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Acknowledgement

The Authors are thankful to the principal of Rama Medical College, Hospital and Research Centre, Kanpur (India) for the permission to do this original research work at this institute and for the financial support.

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Authors and Affiliations

  1. Department of Biochemistry, Rama Medical College and Research Centre, Mandhana, Kanpur, UP, India

    Kahkashan Naaz

  2. Central Research Laboratory, Rama Medical College Hospital and Research Centre, Mandhana, Kanpur, UP, India

    Anil Kumar

  3. Department of Biochemistry, Dr VRK Women’s Medical College, Hyderabad, India

    Ipsita Choudhury

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  1. Kahkashan Naaz
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  2. Anil Kumar
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  3. Ipsita Choudhury
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Correspondence to Ipsita Choudhury.

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Naaz, K., Kumar, A. & Choudhury, I. Assessment of FTO Gene Polymorphism and its Association with Type 2 Diabetes Mellitus in North Indian Populations. Ind J Clin Biochem 34, 479–484 (2019). https://doi.org/10.1007/s12291-018-0778-2

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  • DOI: https://doi.org/10.1007/s12291-018-0778-2

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