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Immuno-Modulatory Effect and Therapeutic Potential of Brugia malayi Cystatin in Experimentally Induced Arthritis

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Abstract

Helminths are known to modulate host’s immune system and understanding this modulation can help in identification of novel therapeutic agents for autoimmune diseases. In this study, we have assessed the immune-modulatory activity and the therapeutic effect of Brugia malayi recombinant cystatin (rBmCys) in methylated BSA (mBSA) induced arthritis using rodent model. Administration of rBmCys has suppressed the severity of mBSA-arthritis in mastomys by reducing paw swelling and other clinical disease parameters as evident from significantly decreased arthritic index. The anti-arthritic effect of rBmCys was also confirmed by decreased histopathological score for synovitis, bone erosion and fibrosis in the tissue sections of paws. Further, this therapeutic effect of cystatin was found to be associated with significantly decreased production of IFN-γ and TNF-α and increased release of IL-4 and IL-10 cytokines. These results implied that rBmCys treatment has alleviated mBSA-induced arthritis and thus can be a promising alternative agent for the treatment of arthritis.

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Acknowledgments

The financial support by Department of Biotechnology (DBT), Ministry of Science & Technology, Government of India through research project (Project Id. BT/PR/4988/INF/22/155/2012) is gratefully acknowledged.

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Authors and Affiliations

  1. Department of Biochemistry & JB Tropical Disease Research Center, Mahatma Gandhi Institute of Medical Sciences, Sevagram, Maharashtra, 442 102, India

    Ravi Shankar Prasad Yadav, Vishal Khatri, Nitin Amdare, Kalyan Goswami & Maryada Venkata Rami Reddy

  2. Department of Pathology, Mahatma Gandhi Institute of Medical Sciences, Sevagram, Maharashtra, 442 102, India

    V. B. Shivkumar & Nitin Gangane

Authors
  1. Ravi Shankar Prasad Yadav
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  2. Vishal Khatri
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  3. Nitin Amdare
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  4. Kalyan Goswami
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  5. V. B. Shivkumar
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  7. Maryada Venkata Rami Reddy
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Correspondence to Maryada Venkata Rami Reddy.

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All the authors have declared that they have no conflict of interest.

Ethical Approval

This study does not contain any studies with human participants performed by any of the authors. This study was approved by Institutional Animal Ethics Committee and national guidelines as per Committee for the Purpose of Control and Supervision of Experiments on Animals (CPCSEA) for the care and use of animals were followed.

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Yadav, R.S.P., Khatri, V., Amdare, N. et al. Immuno-Modulatory Effect and Therapeutic Potential of Brugia malayi Cystatin in Experimentally Induced Arthritis. Ind J Clin Biochem 31, 203–208 (2016). https://doi.org/10.1007/s12291-015-0515-z

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  • DOI: https://doi.org/10.1007/s12291-015-0515-z

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