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Determination of PON1 Gene Polymorphisms in Iranian Individuals with High LDL/HDL Ratios

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Abstract

Elevated LDL/HDL ratio is an important risk factor for predicting atherosclerosis. Paraoxonase-1 protects LDLs from oxidative modifications and has a protective effect against atherosclerosis. Two common polymorphisms, Q192R and L55M, in PON1 gene can affect PON1 levels and function. The aim of this study was to evaluate the frequency PON1 polymorphisms in individuals with high and normal LDL/HDL ratios. To evaluate Q192R and L55M polymorphisms in Iranian case group (n = 70) with high LDL/HDL ratio, and control group (n = 80) with normal LDL/HDL ratio, we used PCR–RFLP method. Genotype frequencies for Q192R were 52.2 % for QQ, 46.3 % for QR, and 1.5 % for RR in case group (P = 0.003). However, Genotype frequencies in case group for L55M were 17.5 % LL, 75.3 % LM, and 7.2 % MM (P = 0.001). PON1 L55M polymorphism was associated with high LDL/HDL ratios in case group. So, the L55M polymorphisms can contribute in reducing the antioxidant function and decreasing level of HDL particles. In conclusion, PON1 L55M polymorphism can affect lipid metabolism and may be related to atherosclerosis in Iranian individuals.

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Acknowledgments

This study was financially supported by the Zanjan Metabolic Disorders Research Center. We also appreciate to Endocrinology and Metabolism Research Center of Shariati Hospital, Tehran.

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Authors and Affiliations

  1. Department of Molecular Sciences, School of Medicine, North Khorasan University of Medical Sciences, Bojnurd, Iran

    Amirhosein Khoshi

  2. Department of Pathology and Molecular Medicine, Zanjan University of Medical Sciences, Zanjan, Iran

    Yousef Mortazavi & Saeideh Mazloomzadeh

  3. Department of Cardiology, Zanjan University of Medical Sciences, Zanjan, Iran

    Sepideh Sokhanvar

  4. Department of Parasitology, Mazandaran University of Medical Sciences, Sari, Iran

    Asal Tanzifi

  5. Department of Clinical Biochemistry, Zanjan University of Medical Sciences, Zanjan, Iran

    Abbas Akbari & Sadraddin Kalantari

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  1. Amirhosein Khoshi
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Correspondence to Amirhosein Khoshi.

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Khoshi, A., Mortazavi, Y., Sokhanvar, S. et al. Determination of PON1 Gene Polymorphisms in Iranian Individuals with High LDL/HDL Ratios. Ind J Clin Biochem 30, 449–456 (2015). https://doi.org/10.1007/s12291-015-0479-z

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  • DOI: https://doi.org/10.1007/s12291-015-0479-z

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