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Urinary Glycosaminoglycan Estimation as a Routine Clinical Service

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Indian Journal of Clinical Biochemistry Aims and scope Submit manuscript

Abstract

Mucopolysaccharidoses, a group of inherited disorders are associated with defects in glycosaminoglycan metabolism. Thus, assessment of urinary glycosaminoglycan is used as a screening test for mucopolysaccharidoses. The detection methods range from qualitative spot tests to quantification using metachromatic dyes. In our laboratory we optimized a spectrophotometric quantitative method using a metachromatic dye, dimethylmethylene blue. Heparan sulfate was used for quantification. The glycosaminoglycan–dye complex showed a marked shift in color with increase in concentration. The color complex was quantified at 520 nm. The method was linear from 10–89 mg/L. An age matched normal range was obtained in 177 healthy individuals, grouped in 8 different age groups from neonates to adults. Urinary glycosaminoglycan concentration varied distinctly amongst the study population wherein the lowest range in healthy neonates was more than 3 times the upper limit of healthy adults. Urine samples from 10 patients with mucopolysaccharidoses were also included in the study for clinical validation. The method qualified both analytical and clinical validation and was found to be simple, robust and ideal to be offered as a screening test for mucopplysaccharidoses in a routine clinical chemistry laboratory.

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Acknowledgments

We acknowledge support extended by the National Health & Education society for the funding, healthy volunteers for the study sample. Dr. Dhanashri Shetty for reviewing the manuscript and Dr. Vrajesh Udani for referring MPS patients for validation.

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Authors and Affiliations

  1. Biochemistry Department, P.D.Hinduja National Hospital & Medical Research Centre, T.H. Kataria Marg, Mumbai, 400 016, India

    M. B. Dave, P. K. Chawla, A. J. Dherai & T. F. Ashavaid

Authors
  1. M. B. Dave
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  2. P. K. Chawla
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  3. A. J. Dherai
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  4. T. F. Ashavaid
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Correspondence to A. J. Dherai.

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Dave, M.B., Chawla, P.K., Dherai, A.J. et al. Urinary Glycosaminoglycan Estimation as a Routine Clinical Service. Ind J Clin Biochem 30, 293–297 (2015). https://doi.org/10.1007/s12291-014-0456-y

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  • DOI: https://doi.org/10.1007/s12291-014-0456-y

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