Skip to main content

Advertisement

SpringerLink
Log in

One-step nucleic acid amplification assay (OSNA) for sentinel lymph node biopsy

  • Special Feature
  • Recent advances in sentinel node biopsy for breast cancer
  • Published:
Breast Cancer Aims and scope Submit manuscript

Abstract

The one-step nucleic acid amplification assay (OSNA) has been developed for the intraoperative rapid detection of sentinel lymph node metastasis. Pooled analysis of recent studies comparing OSNA with pathology indicated that OSNA is as accurate as pathology (96.3 % concordance rate) and is useful for making the decision to omit axillary dissection for OSNA-negative patients (97.4 % negative predictive value). The advantage of OSNA over pathology is that the former allows the semiquantitative evaluation of total tumor volume in the node when a whole node is examined. OSNA is expected to be a powerful tool for the estimation of risk of non-sentinel lymph node metastasis and also patient prognosis, though further studies about this issue with larger numbers of patients is needed.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Tsujimoto M, Nakabayashi K, Yoshidome K, Kaneko T, Iwase T, Akiyama F, et al. One-step nucleic acid amplification for intraoperative detection of lymph node metastasis in breast cancer patients. Clin Cancer Res. 2007;13:4807–16.

    Article  CAS  PubMed  Google Scholar 

  2. Giuliano AE, Hunt KK, Ballman KV, Beitsch PD, Whitworth PW, Blumencranz PW, et al. Axillary dissection vs no axillary dissection in women with invasive breast cancer and sentinel node metastasis: a randomized clinical trial. JAMA. 2011;305:569–75.

    Article  CAS  PubMed  Google Scholar 

  3. Giuliano AE, Hawes D, Ballman KV, Whitworth PW, Blumencranz PW, Reintgen DS, et al. Association of occult metastases in sentinel lymph nodes and bone marrow with survival among women with early-stage invasive breast cancer. JAMA. 2011;306:385–93.

    Article  CAS  PubMed  Google Scholar 

  4. Visser M, Jiwa M, Horstman A, Brink AA, Pol RP, van Diest P, et al. Intra-operative rapid diagnostic method based on CK19 mRNA expression for the detection of lymph node metastases in breast cancer. Int J Cancer. 2008;122:2562–7.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  5. Schem C, Maass N, Bauerschlag DO, Carstensen MH, Löning T, Roder C, et al. One-step nucleic acid amplification—a molecular method for the detection of lymph node metastases in breast cancer patients; results of the German study group. Virchows Arch. 2009;454:203–10.

    Article  PubMed  Google Scholar 

  6. Tamaki Y, Akiyama F, Iwase T, Kaneko T, Tsuda H, Sato K, et al. Molecular detection of lymph node metastases in breast cancer patients: results of a multicenter trial using the one-step nucleic acid amplification assay. Clin Cancer Res. 2009;15:2879–84.

    Article  CAS  PubMed  Google Scholar 

  7. Feldman S, Krishnamurthy S, Gillanders W, Gittelman M, Beitsch PD, Young PR, et al. A novel automated assay for the rapid identification of metastatic breast carcinoma in sentinel lymph nodes. Cancer. 2011;117:2599–607.

    Article  CAS  PubMed  Google Scholar 

  8. Khaddage A, Berremila SA, Forest F, Clemenson A, Bouteille C, Seffert P, et al. Implementation of molecular intra-operative assessment of sentinel lymph node in breast cancer. Anticancer Res. 2011;31:585–90.

    PubMed  Google Scholar 

  9. Snook KL, Layer GT, Jackson PA, de Vries CS, Shousha S, Sinnett HD, et al. Multicentre evaluation of intraoperative molecular analysis of sentinel lymph nodes in breast carcinoma. Br J Surg. 2011;98:527–35.

    Article  CAS  PubMed  Google Scholar 

  10. Le Frère-Belda MA, Bats AS, Gillaizeau F, Poulet B, Clough KB, Nos C, et al. Diagnostic performance of one-step nucleic acid amplification for intraoperative sentinel node metastasis detection in breast cancer patients. Int J Cancer. 2011;130:2377–86.

    Article  PubMed  Google Scholar 

  11. Bernet L, Cano R, Martinez M, Dueñas B, Matias-Guiu X, Morell L, Palacios J, et al. Diagnosis of the sentinel lymph node in breast cancer: a reproducible molecular method: a multicentric Spanish study. Histopathology. 2011;58:863–9.

    Article  PubMed  Google Scholar 

  12. Sagara Y, Ohi Y, Matsukata A, Yotsumoto D, Baba S, Tamada S, Sagara Y, et al. Clinical application of the one-step nucleic acid amplification method to detect sentinel lymph node metastasis in breast cancer. Breast Cancer. 2012 (in press).

  13. Tamaki Y, Sato N, Homma K, Takabatake D, Nishimura R, Tsujimoto M, et al. Routine clinical use of the one-step nucleic acid amplification assay for detection of sentinel lymph node metastases in breast cancer patients: result of a multicenter study in Japan. Cancer. 2012 (in press).

  14. Godey F, Leveque J, Tas P, Gandon G, Poree P, Mesbah H, et al. Sentinel lymph node analysis in breast cancer: contribution of one-step nucleic acid amplification (OSNA). Breast Cancer Res Treat. 2012;131:509–16.

    Article  PubMed  Google Scholar 

  15. Vogué LB, Rojo F, Hardisson D, Iturriagagoitia AC, Panadés MJ, Velasco A, et al. Comparison of molecular analysis and histopathology for axillary lymph node staging in primary breast cancer: results of the B-CLOSER-1 study. Diagn Mol Pathol. 2012;21:69–76.

    Article  Google Scholar 

  16. Osako T, Iwase T, Kimura K, Yamashita K, Horii R, Yanagisawa A, et al. Intraoperative molecular assay for sentinel lymph node metastases in early stage breast cancer: a comparative analysis between one-step nucleic acid amplification whole node assay and routine frozen section histology. Cancer. 2011;117:4365–74.

    Article  CAS  PubMed  Google Scholar 

  17. Castellano I, Marci L, Deambrogio C, Balmativola D, Bussone R, Ala A, et al. Reliability of whole sentinel lymph node analysis by one-step nucleic acid amplification for intraoperative diagnosis of breast cancer metastases. Ann Surg. 2012;255:334–42.

    Article  PubMed  Google Scholar 

  18. Viale G, Maiorano E, Pruneri G, Mastropasqua MG, Valentini S, Galimberti V, et al. Predicting the risk for additional axillary metastases in patients with breast carcinoma and positive sentinel lymph node biopsy. Ann Surg. 2005;241:319–25.

    Article  PubMed Central  PubMed  Google Scholar 

  19. Christiansen P, Friis E, Balslev E, Jensen D, Møller S, Danish Breast Cancer Cooperative Group. Sentinel node biopsy in breast cancer: five years experience. 2008;47:561–8.

  20. Chu PG, Weiss LM. Keratin expression in human tissues and neoplasms. Histopathology. 2002;40:403–39.

    Article  CAS  PubMed  Google Scholar 

  21. Parikh RR, Yang Q, Higgins SA, Haffty BG. Outcomes in young women with breast cancer of triple-negative phenotype: the prognostic significance of CK 19 expression. Int J Radiat Oncol Biol Phys. 2008;70:35–42.

    Article  PubMed  Google Scholar 

  22. Viardell F, Novell A, Martin J, Santacana M, Velasco A, Diez-Castro MJ, et al. Importance of assessing CK19 immunostaining in core biopsies in patients subjected to sentinel node biopsy by OSNA. Virchows Arch. 2012 (in press).

  23. de Boer M, van Dijck JA, Bult P, Borm GF, Tjan-Heijnen VC. Breast cancer prognosis and occult lymph node metastases, isolated tumor cells, and micrometastases. J Natl Cancer Inst. 2010;102:410–25.

    Article  PubMed  Google Scholar 

  24. de Boer M, van Deurzen CH, van Dijck JA, Borm GF, van Diest PJ, Adang EM, et al. Micrometastases or isolated tumor cells and the outcome of breast cancer. N Engl J Med. 2009;361:653–63.

    Article  PubMed  Google Scholar 

  25. Reed J, Rosman M, Verbanac KM, Mannie A, Cheng Z, Tafra L. Prognostic implications of isolated tumor cells and micrometastases in sentinel nodes of patients with invasive breast cancer: 10-year analysis of patients enrolled in the prospective East Carolina University/Anne Arundel Medical Center Sentinel Node Multicenter Study. J Am Coll Surg. 2009;208:333–40.

    Article  PubMed  Google Scholar 

  26. Hansen NM, Grube B, Ye X, Turner RR, Brenner RJ, Sim MS, et al. Impact of micrometastases in the sentinel node of patients with invasive breast cancer. J Clin Oncol. 2009;27:4679–84.

    Article  PubMed  Google Scholar 

  27. Gobardhan PD, Elias SG, Madsen EVE, van Wely B, van den Wildenberg F, Theunissen EBM, et al. Prognostic value of lymph node micrometastases in breast cancer: a multicenter cohort study. Ann Surg Oncol. 2011;18:1657–64.

    Article  PubMed Central  PubMed  Google Scholar 

  28. Weaver DL, Ashikaga T, Krag DN, Skelly JM, Anderson SJ, Harlow SP, et al. Effect of occult metastases on survival in node-negative breast cancer. New Engl J Med. 2011;364:412–21.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  29. Pepels MJ, de Boer M, Bult P, van Dijck JA, van Deurzen CH, Menke-Pluymers MB, et al. Regional recurrence in breast cancer patients with sentinel node micrometastases and isolated tumor cells. Ann Surg. 2012;255:116–21.

    Article  PubMed  Google Scholar 

  30. Giuliano AE, McCall LM, Beitsch P, Whitworth PW, Blumencrantz P, Leitch M, et al. Locoregional recurrence after sentinel lymph node dissection with or without axillary dissection in patients with sentinel lymph node metastases. Ann Surg. 2010;252:426–33.

    PubMed  Google Scholar 

  31. Rebollo-Aguirre AC, Gallego-Peinado M, Menjón-Beltrán S, Garcia–Garcia J, Pastor-Pons E, Chamorro-Santos CE, et al. Sentinel lymph node biopsy in patients with operable breast cancer treated with neoadjuvant chemotherapy. Rev Esp Med Nucl. 2012;31:117–23.

    CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Breast and Endocrine Surgery, Osaka Medical Center for Cancer and Cardiovascular Diseases, 1-3-3, Nakamichi, Higashinari-ku, Osaka, 537-8511, Japan

    Yasuhiro Tamaki

Authors
  1. Yasuhiro Tamaki
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Yasuhiro Tamaki.

About this article

Cite this article

Tamaki, Y. One-step nucleic acid amplification assay (OSNA) for sentinel lymph node biopsy. Breast Cancer 22, 230–234 (2015). https://doi.org/10.1007/s12282-012-0390-x

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12282-012-0390-x

Keywords

Search

Navigation