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Experimental phasing using zinc and sulfur anomalous signals measured at the zinc absorption peak

  • Microbial Physiology and Biochemistry
  • Published:
Journal of Microbiology Aims and scope Submit manuscript

An Author’s Correction to this article was published on 08 November 2013

Abstract

Iron is an essential transition metal required for bacterial growth and survival. Excess free iron can lead to the generation of reactive oxygen species that can cause severe damage to cellular functions. Cells have developed iron-sensing regulators to maintain iron homeostasis at the transcription level. The ferric uptake regulator (Fur) is an iron-responsive regulator that controls the expression of genes involved in iron homeostasis, bacterial virulence, stress resistance, and redox metabolism. Here, we report the expression, purification, crystallization, and phasing of the apo-form of Bacillus subtilis Fur (BsFur) in the absence of regulatory metal ions. Crystals were obtained by microbatch crystallization method at 295 K and diffraction data at a resolution of 2.6 Å was collected at the zinc peak wavelength (λ=1.2823 Å). Experimental phasing identified the positions of one zinc atom and four sulfur atoms of cysteine residues coordinating the zinc atom, indicating that the data contained a meaningful anomalous scattering originating from the ordered zinc-coordinating sulfur atoms, in spite of the small anomalous signals of sulfur atoms at the examined wavelength.

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Authors and Affiliations

  1. Marine Biotechnology Research Division, Korea Institute of Ocean Science and Technology, Ansan, 426-744, Republic of Korea

    Sangmin Lee, Min-Kyu Kim & Sun-Shin Cha

  2. Ocean Science and Technology School, Korea Maritime University, Pusan, 606-791, Republic of Korea

    Sangmin Lee & Sun-Shin Cha

  3. Department of Life Science and Institute for Natural Sciences, Hanyang University, Seoul, 133-791, Republic of Korea

    Chang-Jun Ji & Jin-Won Lee

  4. Department of Marine Biotechnology, University of Science and Technology, Daejeon, 305-333, Republic of Korea

    Sun-Shin Cha

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  1. Sangmin Lee
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  2. Min-Kyu Kim
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  4. Jin-Won Lee
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Correspondence to Jin-Won Lee or Sun-Shin Cha.

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Lee, S., Kim, MK., Ji, CJ. et al. Experimental phasing using zinc and sulfur anomalous signals measured at the zinc absorption peak. J Microbiol. 51, 639–643 (2013). https://doi.org/10.1007/s12275-013-3412-2

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  • DOI: https://doi.org/10.1007/s12275-013-3412-2

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