Antioxidant capacity of novel pigments from an Antarctic bacterium
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In Antarctica microorganisms are exposed to several conditions that trigger the generation of reactive oxygen species, such as high UV radiation. Under these conditions they must have an important antioxidant defense system in order to prevent oxidative damage. One of these defenses are pigments which are part of the non-enzymatic antioxidant mechanisms. In this work we focused on the antioxidant capacity of pigments from an Antarctic microorganism belonging to Pedobacter genus. This microorganism produces different types of pigments which belong to the carotenoids group. The antioxidant capacity of a mix of pigments was analyzed by three different methods: 1,1-diphenyl-2-picrylhydrazyl, ROS detection and oxygen electrode. The results obtained from these approaches indicate that the mix of pigments has a strong antioxidant capacity. The oxidative damage induced by UVB exposure to liposomes was also analyzed. Intercalated pigments within the liposomes improved its resistance to lipid peroxidation. Based on the analysis carried out along this research we conclude that the antioxidant properties of the mix of pigments protect this bacterium against oxidative damage. These properties make this mix of pigments a powerful antioxidant mixture with potential biotechnological applications.
- Asker, D. and Ohta, Y. 1999. Production of canthaxanthin by extremely halophilic bacteria. J. Biosci. Bioeng. 8, 617–621. CrossRef
- Bergey, D.H., Holt, J., Krieg, N., and Sneath, P. 1994. Bergey’s manual of determinative bacteriology. 9th (ed.). Lippincott Williams & Wilkins.
- Bondet, V., Brand-Williams, W., and Berset, C. 1997. Kinetics and mechanisms of antioxidant activity using the DPPH·free radical method. Food Sci. Technol. 330, 609–615.
- Brand-Williams, W., Cuvelier, M., and Berset, C. 1995. Use of a free radical method to evaluate antioxidant activity. Food Sci. Technol. 28, 25–30.
- Briviba, K., Klotz, L., and Sies, H. 1997. Toxic and signaling effects of photochemically or chemically generated singlet oxygen in biological systems. Biol. Chem. 378, 1259–1265.
- Carpenter, E.J., Lin, S., and Capone, D.G. 2000. Bacterial activity in south pole snow. Appl. Environ. Microbiol. 66, 4514–4517. CrossRef
- Chappell, J.B. 1964. The oxidation of citrate isocitrate and cis-aconitate by isolated mitochondria. Biochem. J. 90, 225–237.
- Chattopadhyay, M.K., Jagannadham, M.V., Vairamani, M., and Shivaji, S. 1997. Carotenoid pigments of a antarctic psychrotrophic bacterium Micrococcus roseus: temperature dependent biosynthesis, structure, and interaction with synthetic membranes. Biochem. Biophys. Res. Commun. 239, 85–90. CrossRef
- Chintalapati, S., Kiran, M.D., and Shiva, I.S. 2004. Role of membrane lipid fatty acids in cold adaptation. Cell Mol. Biol. 50, 631–642.
- Cubillos, M., Lissi, E., and Abuin, E. 2000. Kinetics of lipid peroxidation in compartmentalized systems initiated by a water-soluble free radical source. Chem. Phys. Lipids 104, 49–56. CrossRef
- De Rosso, V.V. and Mercadante, A.Z. 2007. Identification and quantification of carotenoids, by HPLC-PDA-MS/MS, from Amazonian fruits. J. Agric. Food Chem. 55, 5062–5072. CrossRef
- Dundas, I.D. and Larsen, H. 1962. The physiological role of the carotenoid pigments of Halobacterium salinarium. Arch. Microbiol. 44, 233–239.
- Estevez, M.S., Malanga, G., and Puntarulo, S. 2001. UV-B effects on Antarctic Chlorella sp. cells. J. Photochem. Photobiol. 62, 19–25. CrossRef
- Feller, G. and Gerday, C. 2003. Psychrophilic enzymes: hot topics in cold adaptation. Nat. Rev. Microbiol. 1, 200–208. CrossRef
- Fong, N.J., Burgess, M.L., Barrow, K.D., and Glenn, D.R. 2001. Carotenoid accumulation in the psychrotrophic bacterium Arthrobacter agilis in response to thermal and salt stress. Appl. Microbiol. Biotechnol. 56, 750–756. CrossRef
- Friedmann, E.I. 1982. Endolithic microorganisms in the Antarctic cold desert. Science 215, 1045–1053. CrossRef
- Gochnauer, M.B., Kushwaha, S.C., Kates, M., and Kushner, D.J. 1972. Nutritional control of pigment and isoprenoid compound formation in extremely halophilic bacteria. Arch. Microbiol. 84, 339–349.
- Halliwell, B. 2006. Reactive species and antioxidants. Redox biology is a fundamental theme of aerobic life. Plant Physiol. 141, 312–322. CrossRef
- Imlay, J. 2003. Pathways of oxidative damage. Annu. Rev. Microbiol. 57, 395–418. CrossRef
- Jagannadham, M.V., Rao, V.J., and Shivaji, S. 1991. The major carotenoid pigment of a psychrotrophic Micrococcus roseus strain: purification, structure, and interaction with synthetic membranes. J. Bacteriol. 173, 7911–7917.
- Johnson, J.L. 1991. Isolation and purification of nucleic acids. pp. 1–19. In Stackebrandt, E. and Goodfellow, M. (eds), Nucleic Acid Techniques in Bacterial Systematics, John Wiley & Sons, Chichester, UK.
- Molyneux, P. 2004. The use of the stable free radical diphenylpicrylhydrazyl (DPPH) for estimating antioxidant activity. Songklanakarin J. Sci. Technol. 26, 211–219.
- Morita, R.Y. 1975. Psychrophilic bacteria. Bacteriol. Rev. 39, 144–167.
- Reysenbach, A.L. 2001. Microbiology of ancient and modern hydrothermal systems. Trends Microbiol. 9, 79–86. CrossRef
- Rodriguez-Amaya, D.B., Raymundo, L.C., Lee, T.C., Simpson, K.L., and Chichester, C.O. 1976. Carotenoid pigment changes in ripening Momordica charantia fruits. Ann. Bot. 40, 615–624.
- Smith, M.C., Prezelin, B.B., Baker, K.S., Bidigare, R.R., Boucher, N.P., Coley, T., Karentz, D., Macintyre, S., Matlick, H.A., Menzies, D., and et al. 1992. Ozone depletion: ultraviolet radiation and phytoplankton biology in antarctic waters. Science 255, 952–959. CrossRef
- Sujak, A., Gabrielska, J., Grudzecki, W., Borc, R., Mazurek, P., and Gruszecki, W.I. 1999. Lutein and zeaxanthin as protectors of lipid membranes against oxidative damage: the structural aspects. Arch. Biochem. Biophys. 371, 301–307. CrossRef
- Suresh, P., Ghosh, M., Pulicherla, K.K., and Sambasiva Rao, K.R.S. 2011. Cold active enzymes from the marine psychrophiles: Biotechnological perspective. Adv. Biotech 10, 16–20.
- Tanner, M.A., Coleman, W.J., Yang, M.M., and Youvan, D.C. 2000. Complex microbial communities inhabiting sulfide-rich black mud from marine coastal environments. Biotech. et alia 8, 1–16.
- Wu, H., Gao, K., Villafane, V.E., Watanabe, T., and Helbling, E.W. 2005. Effects of solar UV radiation on morphology and photosynthesis of filamentous Cyanobacterium. Appl. Environ. Microbiol. 71, 5004–5013. CrossRef
- Zhang, D.H., Lee, Y.K., Ng, M.L., and Phang, S.M. 1997. Composition and accumulation of secondary carotenoids in Chorococcum sp. J. Appl. Phycol. 9, 147–155. CrossRef
- Antioxidant capacity of novel pigments from an Antarctic bacterium
Journal of Microbiology
Volume 50, Issue 3 , pp 374-379
- Cover Date
- Print ISSN
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- The Microbiological Society of Korea
- Additional Links
- antioxidant capacity assays
- Industry Sectors
- Author Affiliations
- 1. Scientific and Cultural Bioscience Foundation, Jose Domingo Canas, 2280, Santiago, Chile
- 2. Doctorate of Biotechnology, University of Santiago of Chile, Av. Libertador Bernardo O Higgins 3363, Santiago, Chile