Isolation and characterization of plant growth-promoting rhizobacteria from wheat roots by wheat germ agglutinin labeled with fluorescein isothiocyanate
Purchase on Springer.com
$39.95 / €34.95 / £29.95*
Rent the article at a discountRent now
* Final gross prices may vary according to local VAT.
Thirty-two isolates were obtained from wheat rhizosphere by wheat germ agglutinin (WGA) labeled with fluorescein isothiocyanate (FITC). Most isolates were able to produce indole acetic acid (65.6%) and siderophores (59.3%), as well as exhibited phosphate solubilization (96.8%). Fourteen isolates displayed three plant growth-promoting traits. Among these strains, two phosphate-dissolving ones, WS29 and WS31, were evaluated for their beneficial effects on the early growth of wheat (Triticum aestivum Wan33). Strain WS29 and WS31 significantly promoted the development of lateral roots by 34.9% and 27.6%, as well as increased the root dry weight by 25.0% and 25.6%, respectively, compared to those of the control. Based on 16S rRNA gene sequence comparisons and phylogenetic positions, both isolates were determined to belong to the genus Bacillus. The proportion of isolates showing the properties of plant growth-promoting rhizobacteria (PGPR) was higher than in previous reports. The efficiency of the isolation of PGPR strains was also greatly increased by WGA labeled with FITC. The present study indicated that WGA could be used as an effective tool for isolating PGPR strains with high affinity to host plants from wheat roots. The proposed approach could facilitate research on biofertilizers or biocontrol agents.
- Abbasi, M.K., Sharif, S., Kazmi, M., Sultan, T., and Aslam, M. 2011. Isolation of plant growth promoting rhizobacteria from wheat rhizosphere and their effect on improving growth, yield and nutrient uptake of plants. Plant Biosyst. 145, 159–168. CrossRef
- Alagawadi, A. and Gaur, A. 1988. Associative effect of Rhizobium and phosphate-solubilizing bacteria on the yield and nutrient uptake of chickpea. Plant Soil 105, 241–246. CrossRef
- Antonyuk, L.P. and Evseeva, N.V. 2006. Wheat lectin as a factor in plant-microbial communication and a stress response protein. Microbiology 75, 470–475. CrossRef
- Antonyuk, L.P. and Ignatov, V.V. 2001. The role of wheat germ agglutinin in plant-bacteria interactions: A hypothesis and the evidence in its support. Russ. J. Plant. Physl. 48, 364–369. CrossRef
- Aub, J.C., Sanford, B.H., and Wang, L.H. 1965. Reactions of normal and leukemic cell surfaces to a wheat germ agglutinin. Proc. Natl. Acad. Sci. USA 54, 400. CrossRef
- Bano, N. and Musarrat, J. 2003. Characterization of a new Pseudomonas aeruginosa strain NJ-15 as a potential biocontrol agent. Curr. Microbiol. 46, 0324–0328. CrossRef
- Bashan, Y. 1998. Inoculants of plant growth-promoting bacteria for use in agriculture. Biotechnol. Adv. 16, 729–770. CrossRef
- Behbahani, M. 2010. Investigation of biological behavior and colonization ability of Iranian indigenous phosphate solubilizing bacteria. Sci. Hortic. 124, 393–399. CrossRef
- Bohlool, B.B. and Schmidt, E.L. 1974. Lectins: A possible basis for specificity in the rhizobium-legume root nodule symbiosis. Science 185, 269–271. CrossRef
- Burger, M.M. and Goldberg, A.R. 1967. Identification of a tumor-specific determinant on neoplastic cell surfaces. Proc. Natl. Acad. Sci. USA 57, 359. CrossRef
- Cattelan, A., Hartel, P., and Fuhrmann, J. 1999. Screening for plant growth-promoting rhizobacteria to promote early soybean growth. Soil Sci. Soc. Am. J. 63, 1670. CrossRef
- Chopade, B.A., Sachdev, D.P., Chaudhari, H.G., Kasture, V.M., and Dhavale, D.D. 2009. Isolation and characterization of indole acetic acid (IAA) producing Klebsiella pneumoniae strains from rhizosphere of wheat (Triticum aestivum) and their effect on plant growth. Indian. J. Exp. Biol. 47, 993–1000.
- De Hoff, P., Brill, L., and Hirsch, A. 2009. Plant lectins: the ties that bind in root symbiosis and plant defense. Mol. Genet. Genom. 282, 1–15. CrossRef
- Del Gallo, M. and Fendrik, I. 1994. The rhizosphere and Azospirillum. pp. 57–75. In Okon, Y. (ed.) Azospirillum/Plant Associations. C.R.C. Press, Boca Raton, FL, USA.
- Dhandapani. 2011. Insoluble phosphate solubilization by bacterial strains isolated from rice rhizosphere soils from southern India. Int. J. Soil Sci. 6, 134–141. CrossRef
- Ding, Y., Wang, J., Liu, Y., and Chen, S. 2005. Isolation and identification of nitrogen-fixing Bacilli from plant rhizospheres in Beijing region. J. Appl. Microbiol. 99, 1271–1281. CrossRef
- Fischer, S.E., Fischer, S.I., Magris, S., and Mori, G.B. 2007. Isolation and characterization of bacteria from the rhizosphere of wheat. World J. Microbiol. Biotechnol. 23, 895–903. CrossRef
- Flores-Vargas, R.D. and O’Hara, G.W. 2006. Isolation and characterization of rhizosphere bacteria with potential for biological control of weeds in vineyards. J. Appl. Microbiol. 100, 946–954. CrossRef
- Germida, J.J., Siciliano, S.D., Renato de Freitas, J., and Seib, A.M. 1998. Diversity of root-associated bacteria associated with field-grown canola (Brassica napus L.) and wheat (Triticum aestivum L.). FEMS Microbiol. Ecol. 26, 43–50. CrossRef
- Glick, B.R. 1995. The enhancement of plant growth by free-living bacteria. Can. J. Microbiol. 41, 109–117. CrossRef
- Gordon, S.A. and Weber, R.P. 1951. Colorimetric estimation of indoleacetic acid. Plant Physiol. 26, 192. CrossRef
- Gull, M., Hafeez, F.Y., Saleem, M., and Malik, K.A. 2004. Phosphorus uptake and growth promotion of chickpea by co-inoculation of mineral phosphate solubilising bacteria and a mixed rhizobial culture. Aust. J. Exp. Agr. 44, 623–628. CrossRef
- Hafeez, F.Y., Yasmin, S., Ariani, D., Rahman, M., Zafar, Y., and Malik, K.A. 2006. Plant growth-promoting bacteria as biofertilizer. Agron. Sustain. Det. 26, 143–150. CrossRef
- Hartmann, A., Schmid, M., Tuinen, D., and Berg, G. 2009. Plant-driven selection of microbes. Plant Soil 321, 235–257. CrossRef
- Hoagland, D.R. and Arnon, D.I. 1950. The water-culture method for growing plants without soil. Circ. Calif. Agric. Exp. Stn. 347, 4–31.
- Holt, J.G., Kreig, N.R., Sneath, P.H.A., Staley, J.T., and Williams, S.T. 1994. Bergey’s Manual of Determinative Bacteriology. Williams and Wilkins, Baltimore, USA.
- Inbar, M. and Sachs, L. 1969. Interaction of the carbohydrate-binding protein concanavalin A with normal and transformed cells. Proc. Natl. Acad. Sci. USA 63, 1418. CrossRef
- Johnsson, L., Hökeberg, M., and Gerhardson, B. 1998. Performance of the Pseudomonas chlororaphis biocontrol agent MA 342 against cereal seed-borne diseases in field experiments. Eur. J. Plant Pathol. 104, 701–711. CrossRef
- Khalid, A., Arshad, M., and Zahir, Z.A. 2004. Screening plant growth-promoting rhizobacteria for improving growth and yield of wheat. J. Appl. Microbiol. 96, 473–480. CrossRef
- Kumar, K., Amaresan, N., Bhagat, S., Madhuri, K., and Srivastava, R. 2010. Isolation and characterization of rhizobacteria associated with coastal agricultural ecosystem of rhizosphere soils of cultivated vegetable crops. World J. Microbiol. Biotechnol. 27, 1625–1632. CrossRef
- Laguerre, G., Allard, M.-R., Revoy, F., and Amarger, N. 1994. Rapid identification of rhizobia by restriction fragment length polymorphism analysis of PCR-amplified 16S rRNA genes. Appl. Environ. Microbiol. 60, 56–63.
- Levine, D., Kaplan, M.J., and Greenaway, P.J. 1972. The purification and characterization of wheat-germ agglutinin. Biochem. J. 129, 847.
- Mishkind, M., Keegstra, K., and Palevitz, B.A. 1980. Distribution of wheat germ agglutinin in young wheat plants. Plant Physiol. 66, 950–955. CrossRef
- Moreira, R.A., Ainouz, I.L., Oliveira, J.T.A., and Cavada, B.S. 1991. Plant lectins, chemical and biological aspects. Mem. Inst. Oswaldo. Cruz. 86, 211–218. CrossRef
- Nathan, S. 2008. Lectins: past, present and future. Biochem. Soc. Trans. 36, 1457–1460. CrossRef
- Olyunina, L., Matskova, Y., Goncharova, T., and Gushina, Y. 2009. Evaluation of thermal resistance of Azotobacter chroococcum 66 using atomic force microscopy. Appl. Biochem. Microb. 45, 38–42. CrossRef
- Park, J.H., Bolan, N., Megharaj, M., and Naidu, R. 2011. Isolation of phosphate solubilizing bacteria and their potential for lead immobilization in soil. J. Hazard. Mater. 185, 829–836. CrossRef
- Park, M., Kim, C., Yang, J., Lee, H., Shin, W., Kim, S., and Sa, T. 2005. Isolation and characterization of diazotrophic growth promoting bacteria from rhizosphere of agricultural crops of Korea. Microbiol. Res. 160, 127–133. CrossRef
- Peumans, W.J. and Damme, E.J.M.V. 1995. Lectins as plant defense proteins. Plant Physiol. 109, 347–352. CrossRef
- Pistole, T.G. 1981. Interaction of bacteria and fungi with lectins and lectin-like substances. Annu. Rev. Microbiol. 35, 85–112. CrossRef
- Schwyn, B. and Neilands, J.B. 1987. Universal chemical assay for the detection and determination of siderophores. Anal. Biochem. 160, 47–56. CrossRef
- Sela, B.A., Lis, H., Sharon, N., and Sachs, L. 1970. Different locations of carbohydrate-containing sites in the surface membrane of normal and transformed mammalian cells. J. Memb. Biol. 3, 267–279. CrossRef
- Senthilkumar, M., Govindasamy, V., and Annapurna, K. 2007. Role of antibiosis in suppression of charcoal rot disease by soybean endophyte Paenibacillus sp. HKA-15. Curr. Microbiol. 55, 25–29. CrossRef
- Tamura, K., Dudley, J., Nei, M., and Kumar, S. 2007. MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Mol. Biol. Evol. 24, 1596–1599. CrossRef
- Vessey, J.K. 2003. Plant growth promoting rhizobacteria as biofertilizers. Plant Soil 255, 571–586. CrossRef
- Weisburg, W.G., Barns, S.M., Pelletier, D.A., and Lane, D.J. 1991. 16S ribosomal DNA amplification for phylogenetic study. J. Bacteriol. 173, 697–703.
- Yegorenkova, I.V., Konnova, S.A., Sachuk, V.N., and Ignatov, V.V. 2001. Azospirillum brasilense colonisation of wheat roots and the role of lectin-carbohydrate interactions in bacterial adsorption and root-hair deformation. Plant Soil 231, 275–282. CrossRef
- Yu, X.M., Ai, C.X., Xin, L., and Zhou, G.F. 2011. The siderophore-producing bacterium, Bacillus subtilis CAS15, has a biocontrol effect on Fusarium wilt and promotes the growth of pepper. Eur. J. Soil Biol. 47, 138–145. CrossRef
- Yuan, C.L., Mou, C.X., Wu, W.L., and Guo, Y.B. 2011. Effect of different fertilization treatments on indole-3-acetic acid producing bacteria in soil. J. Soils Sediments 11, 322–329. CrossRef
- Isolation and characterization of plant growth-promoting rhizobacteria from wheat roots by wheat germ agglutinin labeled with fluorescein isothiocyanate
The Journal of Microbiology
Volume 50, Issue 2 , pp 191-198
- Cover Date
- Print ISSN
- Online ISSN
- The Microbiological Society of Korea
- Additional Links
- FITC-labeled WGA
- Bacillus sp.
- Industry Sectors
- Author Affiliations
- 1. School of Life Science, Anhui Agricultural University, Hefei, 230036, Anhui Province, P. R. China
- 2. Department of Biology, Anhui Science and Technology University, Bengbu, 233100, Anhui Province, P. R. China