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Hierarchical dual-porous hydroxyapatite doped dendritic mesoporous silica nanoparticles based scaffolds promote osteogenesis in vitro and in vivo

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Abstract

Biomaterial based scaffolds for treating large bone defects require excellent biocompatibility and osteoconductivity. Here we report on the fabrication of hydroxyapatite-dendritic mesoporous silica nanoparticles (HA-DMSN) based scaffolds with hierarchical micro-pores (5 µm) and nano-pores (6.4 nm), and their application for bone regeneration. The in vitro studies demonstrated good biocompatibility of dissolution extracts, as well as enhanced osteogenic potential indicated by dose-dependent upregulation of bone marker gene expression (osteocalcin gene (OCN), osteopontin gene (OPN), collagen type I alpha 1 gene (CoL1A1), runt-related transcription factor 2 gene (RUNX2), and integrin-binding sialoprotein gene (IBSP)), alkaline phosphatise (ALP) activity, and alizarin red staining. The in vivo studies showed that HA-DMSN scaffolds significantly increased bone formation in a rat cranial bone defect model after 4 weeks healing. Our study provides a simple method to fabricate promising inorganic scaffolds with hierarchical pores for bone tissue engineering.

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Acknowledgements

The authors acknowledge the support from University of Queensland (UQ) Early Career Researcher Grant (No. 1717673). C. Lei acknowledges the support of Advanced Queensland Fellowship. C. Xu acknowledges the support of National Health & Medical Research Council of Australia (NHMRC) Early Career Fellowship. S. Hosseinpour acknowledges the support of The University of Queensland International (UQI) Scholarship. The authors acknowledge the supports from the Australian Microscopy and Microanalysis Research Facility at the Centre for Microscopy and Microanalysis, the University of Queensland.

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  1. Chang lei and Yuxue Cao contribute equally to this work.

Authors and Affiliations

  1. Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, St. Lucia, QLD, 4072, Australia

    Chang Lei, Yuxue Cao, Fang Gao & Jianye Fu

  2. School of Dentistry, The University of Queensland, Herston, QLD, 4006, Australia

    Yuxue Cao, Sepanta Hosseinpour, Reuben Staples, Saso Ivanovski & Chun Xu

  3. School of Chemistry, Physics and Mechanical Engineering, Science and Engineering Faculty, Queensland University of Technology (QUT), Brisbane, QLD, 4001, Australia

    Jingyu Liu

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  1. Chang Lei
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Correspondence to Saso Ivanovski or Chun Xu.

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12274_2020_3112_MOESM1_ESM.pdf

Hierarchical dual-porous hydroxyapatite doped dendritic mesoporous silica nanoparticles based scaffolds promote osteogenesis in vitro and in vivo

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Lei, C., Cao, Y., Hosseinpour, S. et al. Hierarchical dual-porous hydroxyapatite doped dendritic mesoporous silica nanoparticles based scaffolds promote osteogenesis in vitro and in vivo. Nano Res. 14, 770–777 (2021). https://doi.org/10.1007/s12274-020-3112-2

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