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Magnetic iron oxide nanoparticles accelerate osteogenic differentiation of mesenchymal stem cells via modulation of long noncoding RNA INZEB2

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An Erratum to this article was published on 12 April 2017

Abstract

Nanomaterials are increasingly used for biomedical applications; thus, it is important to understand their biological effects. Previous studies suggested that magnetic iron oxide nanoparticles (IONPs) have tissue-repairing effects. In the present study, we explored cellular effects of IONPs in mesenchymal stem cells (MSCs) and identified the underlying molecular mechanisms. The results showed that our as-prepared IONPs were structurally stable in MSCs and promoted osteogenic differentiation of MSCs as whole particles. Moreover, at the molecular level, we compared the gene expression of MSCs with or without IONP exposure and showed that IONPs upregulated long noncoding RNA INZEB2, which is indispensable for maintaining osteogenesis by MSCs. Furthermore, overexpression of INZEB2 downregulated ZEB2, a factor necessary to repress BMP/Smad-dependent osteogenic transcription. We also demonstrated that the essential role of INZEB2 in osteogenic differentiation was ZEB2-dependent. In summary, we elucidated the molecular basis of IONPs’ effects on MSCs; these findings may serve as a meaningful theoretical foundation for applications of stem cells to regenerative medicine.

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Acknowledgements

This work was supported by grants from the National Basic Research Program of China (Nos. 2013CB733804 and 2013CB934400), the National Natural Science Foundation of China (NSFC) for Key Project of International Cooperation (No. 61420106012), Special Project on Development of National Key Scientific Instruments and Equipment of China (No. 2011YQ03013403), the Natural Science Foundation of Jiangsu Province (No. BK20130608), the Fundamental Research Funds for the Central Universities and the Graduate Research and Innovation Program of Jiangsu Province in China (No. KYLX15-0167).

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Authors and Affiliations

  1. State Key Laboratory of Bioelectronics, Jiangsu Key Laboratory for Biomaterials and Devices, School of Biological Sciences & Medical Engineering, Southeast University, Nanjing, 210096, China

    Qiwei Wang, Bo Chen, Yan Li & Ning Gu

  2. Collaborative Innovation Center of Suzhou Nano Science and Technology, Suzhou, 215123, China

    Qiwei Wang, Bo Chen, Meng Cao & Ning Gu

  3. Key Laboratory of Developmental Genes and Human Diseases in Ministry of Education, Institute of Life Sciences, Southeast University, Nanjing, 210096, China

    Fang Ma

  4. Signalway Antibody LLC, College Park, MD, 20740, USA

    Shikang Lin

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  1. Qiwei Wang
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Correspondence to Ning Gu.

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An erratum to this article is available at http://dx.doi.org/10.1007/s12274-017-1566-7.

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Magnetic iron oxide nanoparticles accelerate osteogenic differentiation of mesenchymal stem cells via modulation of long noncoding RNA INZEB2

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Wang, Q., Chen, B., Ma, F. et al. Magnetic iron oxide nanoparticles accelerate osteogenic differentiation of mesenchymal stem cells via modulation of long noncoding RNA INZEB2 . Nano Res. 10, 626–642 (2017). https://doi.org/10.1007/s12274-016-1322-4

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