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Quantitative measurement of the mechanical properties of human antibodies with sub-10-nm resolution in a liquid environment

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Abstract

The nanomechanical properties of single human immunoglobulin G and M antibodies were measured in a liquid environment using a fast force-volume technique with sub-10-nm spatial resolution. The ultrastructural details of these molecules were resolved in the images. Simultaneously, important physical properties, including elasticity, adhesion, and deformation were measured. The dimensions and adsorption of the immunoglobulin M antibodies onto the substrate indicated that they are highly flexible. The antibodies were characterized by a low elastic stiffness (34 ± 10 MPa) and high deformability (1.5 ± 0.5 nm).

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Authors and Affiliations

  1. Center of Smart Interfaces, Technische Universität Darmstadt, Alarich-Weiss-Str. 10, 64287, Darmstadt, Germany

    Agnieszka Voss, Christian Dietz, Annika Stocker & Robert W. Stark

  2. Physics of Surfaces, Institute of Materials Science, Technische Universität Darmstadt, Alarich-Weiss-Str. 16, 64287, Darmstadt, Germany

    Agnieszka Voss, Christian Dietz, Annika Stocker & Robert W. Stark

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  1. Agnieszka Voss
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  2. Christian Dietz
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Correspondence to Christian Dietz or Robert W. Stark.

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Voss, A., Dietz, C., Stocker, A. et al. Quantitative measurement of the mechanical properties of human antibodies with sub-10-nm resolution in a liquid environment. Nano Res. 8, 1987–1996 (2015). https://doi.org/10.1007/s12274-015-0710-5

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  • DOI: https://doi.org/10.1007/s12274-015-0710-5

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